Tumor Biology

, Volume 36, Issue 12, pp 9763–9767 | Cite as

The key role of astrocyte elevated gene-1 in CCR6-induced EMT in cervical cancer

  • Juan Zhang
  • Dingjun Zhu
  • Qiongying Lv
  • Yuexiong Yi
  • Fei Li
  • Wei Zhang
Research Article


In recent years, astrocyte elevated gene-1 (AEG-1) has been recommended as an important mediator that is involved in the epithelial-to-mesenchymal transition (EMT) process. However, the mechanisms underlying the chemokine (C-C motif) ligand 20 (CCL20)/chemokine (C-C motif) receptor 6 (CCR6)-AEG-1 pathway-mediated EMT in cervical cancer (CC) have not been well featured till now. We used immunohistochemistry and immunoblotting to assess the expression of AEG-1 in 94 cervical cancer tissues and cells. Subsequently, cervical cancer SiHa cells were treated with si-AEG-1 and then subjected to in vitro assays. We observed that AEG-1 proteins were highly expressed in cervical cancer tissues and closely correlated with International Federation of Gynecology and Obstetrics (FIGO) stage and metastasis. Importantly, we validated the expression of AEG-1, p-Erk1/2, p-Akt, vimentin, N-cadherin, and matrix metalloproteinase 2 (MMP2) increased in SiHa with CCL20 treatment in a concentration-dependent manner. When cells were treated with si-AEG-1, the expression of p-Erk1/2, p-Akt, vimentin, N-cadherin, and MMP2 was also downregulated. Using the cell cycle assay, the knockdown of AEG-1 inhibited the entry of G1 into S phase. In conclusion, AEG-1 mediates CCL20/CCR6-induced EMT development via both Erk1/2 and Akt signaling pathway in cervical cancer, which indicates that CCL20/CCR6-AEG-1-EMT pathway could be suggested as a useful target to affect the progression of cervical cancer.


AEG-1 CCR6 EMT Cervical cancer 



We greatly thank the other members of our lab for valuable suggestions and writing.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Juan Zhang
    • 1
  • Dingjun Zhu
    • 1
  • Qiongying Lv
    • 1
  • Yuexiong Yi
    • 1
  • Fei Li
    • 1
  • Wei Zhang
    • 1
  1. 1.The First Department of GynaecologyRenmin Hospital of Wuhan UniversityWuhanChina

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