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Tumor Biology

, Volume 36, Issue 12, pp 9753–9761 | Cite as

Speckle-type POZ protein is negatively associated with malignancies and inhibits cell proliferation and migration in liver cancer

  • Yuping Huang
  • Ning Tan
  • Deshui Jia
  • Ying Jing
  • Qifeng Wang
  • Zhe Li
  • Jiwei Zhang
  • Li Liu
  • Jinjun Li
  • Zhiao Chen
  • Xianghuo He
Research Article

Abstract

Speckle-type POZ protein (SPOP) is an E3 ubiquitin ligase adaptor that is frequently mutated in human cancers. Our previous findings have indicated that SPOP is mutated and functions as a novel tumor suppressor in hepatoblastoma (HB). However, the biological roles and clinical significance of this SPOP in hepatocellular carcinoma (HCC) remain unknown. In this study, we found that the expression level of SPOP was downregulated in HCC primary tumors by quantitative real-time PCR and the protein level of SPOP was also reduced in 72 pairs of HCC tissue microarrays by immunohistochemical analyses. Moreover, SPOP expression was observed to negatively correlate with the tumor grade and intrahepatic metastasis of HCC patients. Furthermore, we revealed that SPOP not only inhibits cell proliferation but also inhibits the motility of liver cancer cells. Finally, we discovered that one of the mechanisms through which SPOP inhibits liver cancer cell migration involves the disruption of ZEB2 expression and the associated epithelial-mesenchymal transition program. Together, our findings emphasize the critical role of SPOP in the regulation of proliferation and migration in liver cancer.

Keywords

SPOP Tumor suppressor Epithelial-mesenchymal transition Liver cancer 

Notes

Acknowledgments

We are grateful for Dr T. Didier’s gifts of the pWPXL, psPAX2, and pMD2.G lenti-virus plasmids. This work was supported by grants from Shanghai Municipal Health Bureau (XBR2011039), Shanghai Natural Science Fund for Youth Scholars (12ZR1449900), and National Natural Science Foundation of China (81201538).

Conflicts of interest

None.

Supplementary material

13277_2015_3753_MOESM1_ESM.docx (1.8 mb)
ESM. 1 (DOCX 1874 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yuping Huang
    • 1
  • Ning Tan
    • 2
  • Deshui Jia
    • 3
  • Ying Jing
    • 1
  • Qifeng Wang
    • 3
  • Zhe Li
    • 3
  • Jiwei Zhang
    • 3
  • Li Liu
    • 3
  • Jinjun Li
    • 1
  • Zhiao Chen
    • 3
  • Xianghuo He
    • 1
    • 3
  1. 1.State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji HospitalShanghai Jiao Tong University School of MedicineShanghaiChina
  2. 2.Guangxi Key Laboratory of Molecular Medicine in Liver Injury and RepairGuilin Medical CollegeGuilinChina
  3. 3.Fudan University Shanghai Cancer Center and Institutes of Biomedical Sciences, Shanghai Medical CollegeFudan UniversityShanghaiChina

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