Antitumor effects evaluation of a novel porphyrin derivative in photodynamic therapy
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In this paper, the antitumor activity of a novel porphyrin-based photosensitizer 5,10,15,20-tetrakis[(5-diethylamino)pentyl] porphyrin (TDPP) was reported in vitro and in vivo. The photophysical and cellular properties of TDPP were investigated. The singlet oxygen generation quantum yield of TDPP was detected; it showed a high singlet oxygen quantum yield of 0.52. The intracellular distribution of photosensitizer was detected with laser scanning confocal microscopy. The efficiency of TDPP-photodynamic therapy (PDT) in vitro was analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and in situ trypan blue exclusion test. Treated with a 630-nm laser, TDPP can kill cultured human esophageal cancer cell line (Eca-109) cells and reduce the growth of Eca-109 xenograft tumors significantly in BABL/c nude mice. And histopathological study was also used to confirm the antitumor effect. It has the perspective to be developed as a new antitumor drug in photodynamic therapy and deserves further investigation.
KeywordsTDPP Porphyrin Tumor Photosensitizer Photodynamic therapy
This work was supported by the Chinese National Natural Science Foundation (No. 21372042, 21402236, 81101298, 81301878), Foundation of Shanghai government (No. 14431906200, 14140903500, 13431900700, 13430722300, 13ZR1441000, 13ZR1440900, 14ZR1439800, 14ZR1439900, 15ZR1439900, 15XD1523400, 14SJGGYY08, 201370), International Cooperation Foundation of China and Croatia (6-11), and Foundation of Yiwu Science and Technology Bureau (No. 2012-G3-02, 2013-G3-03).
Conflicts of interest
- 10.Yan YJ, Zheng MZ, Chen ZL, Yu XH, Yang XX, Ding ZL, et al. Studies on preparation and photodynamic mechanism of chlorin p 6–13,15-N-(cyclohexyl)cycloimide (Chlorin-H) and its antitumor effect for photodynamic therapy in vitro and in vivo. Bioorg Med Chem. 2010;18(17):6282–91. doi: 10.1016/j.bmc.2010.07.027.CrossRefPubMedGoogle Scholar
- 14.Serra AC, Pineiro M, Rocha Gonsalves AM, Abrantes M, Laranjo M, Santos AC, et al. Halogen atom effect on photophysical and photodynamic characteristics of derivatives of 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin. J Photochem Photobiol B Biol. 2008;92(1):59–65. doi: 10.1016/j.jphotobiol.2008.04.006.CrossRefGoogle Scholar
- 17.Juarranz A, Jaen P, Sanz-Rodriguez F, Cuevas J, Gonzalez S. Photodynamic therapy of cancer. Basic principles and applications. Clin Translat Oncology Off Public Fed Spanish Oncol Soc National Cancer Inst of Mexico. 2008;10(3):148–54.Google Scholar