Tumor Biology

, Volume 36, Issue 12, pp 9733–9738 | Cite as

The effect of recombinant lentiviral vector encoding miR-145 on human esophageal cancer cells

  • Tian-Yun Wang
  • Qing-qing Zhang
  • Xi Zhang
  • Qiu-Li Sun
  • Chun-Peng Zhao
  • Xiao-Yin Wang
Research Article


miR-145, a newly identified microRNA molecule, is hypothesized to function as a tumor suppressor, but this activity has not been investigated in esophageal l carcinoma (EC). The aim of this study was to investigate the effect of miR-145 on the biological features of EC cells. miR-145 was obtained using PCR technology and cloned into the lentiviral vector, pLVX-IRES-ZsGreen1, to construct the resulting vector, pLVX-IZ-miR-145. The vector was packaged, the viral titer was tested, and ECA109 cells were infected with the optimal viral titer. Cells that were stably transfected with miR-145 were screened. Flow cytometry was used to analyze enhanced green fluorescence protein gene expression, and to measure cell apoptosis and cell cycle. miR-145 expression was detected by real-time fluorescent quantitative PCR. Furthermore, cell proliferation was assayed using CCK-8 assay. The pLVX-IZ-miR-145 vector was successfully constructed, and the viral titer achieved up to 5.0 × 108 TU/mL. The transfection efficiency was 90 %. Compared to the control group, the expression level of miR-145 in the transfected group was significantly higher (185-fold, P < 0.05). miR-145 overexpression significantly inhibited esophageal cancer cell proliferation (P < 0.05). Moreover, the number of cells at the G2/M stage, as well as the cell apoptotic rate, in the miR-145-transfected group was significantly increased (P < 0.05). Our study reveals that overexpression of miR-145 inhibits cell proliferation, increases apoptosis, and influences the cell cycle progression of EC cell.


MicroRNA Metastasis Apoptosis Esophageal carcinoma Lentiviralvector 



This work was partly supported by grants from the National Natural Science Foundation of China (Nos. 31371332 and 31300702).

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Tian-Yun Wang
    • 1
  • Qing-qing Zhang
    • 2
  • Xi Zhang
    • 1
  • Qiu-Li Sun
    • 1
  • Chun-Peng Zhao
    • 1
  • Xiao-Yin Wang
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyXinxiang Medical UniversityXinxiangChina
  2. 2.Department of Psychosomatic Medicinethe Second Affiliated Hospital of Xinxiang Medical UniversityXinxiangChina

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