Antineoplastic effects of Rhodiola crenulata treatment on B16-F10 melanoma
Melanoma is an aggressive form of skin cancer with limited treatment options for advanced stage disease. Early detection and wide surgical excision remain the initial mode of treatment for primary tumors thus preventing metastases and leading to improved prognosis. Through this work, we have evaluated the antineoplastic effects of Rhodiola crenulata (R. crenulata) root extracts on the B16-F10 melanoma cell line, both in vitro and in vivo. We observed that R. crenulata treatment resulted in increased cell death as well as a reduction in tumor cell proliferation and migration in vitro. Additionally, we observed that R. crenulata decreased the expression of integrin β1 and vimentin and increased the expression of E-cadherin. Further, in mice treated with a topical R. crenulata-based cream therapy, tumors were more likely to have a radial growth pattern, a reduction in mitotic activity, and an increase in tumor necrosis. We also observed that mice drinking water supplemented with R. crenulata displayed a reduction of metastatic foci in disseminated models of melanoma. Collectively, these findings suggest that R. crenulata exhibits striking antitumorigenic and antimetastatic properties and that this extract may harbor potential novel adjuvant therapy for the treatment of melanoma.
KeywordsMelanoma Rhodiola Vimentin Integrin Vertical growth phase Radial growth phase
Vertical growth phase
Radial growth phase
- R. crenulata
This work was graciously supported by the Baystate Summer Student Scholars Program and the University of Massachusetts at Amherst Honors Research Grant. We would like to acknowledge the support, education, and care provided by the Baystate Animal Care Facility for their assistance in animal husbandry for this work.
Conflicts of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- 1.Melanoma of the Skin. In: turning cancer data into discovery. surveillance, epidemiology, and end results (SEER) program. 2004–2010. http://seer.cancer.gov/statfacts/html/melan.html. Accessed 12 March 2015.
- 18.Brown RP, Gerbarg PL. The rhodiola revolution. 1st ed. Holtzbrinck Publishers; 2004.Google Scholar
- 20.Dwyer AV, Whitten DL, Hawrelak JA. Herbal medicines, other than St. John’s Wort, in the treatment of depression: a systematic review. J Clin Ther. 2001;16:40–9.Google Scholar
- 22.Gauger KJ, Rodríguez-Cortés A, Hartwich M, et al. Rhodiola crenulata inhibits the tumorigenic properties of invasive mammary epithelial cells with stem cell characteristics. J Med Plants Res. 2010;4:446–54.Google Scholar
- 28.Skopińska-Różewska E, Hartwich M, Siwicki AK, et al. The influence of Rhodiola rosea extracts and rosavin on cutaneous angiogeneis induced in mice after grafting of syngeneic tumor cells. Cent Eur J Immunol. 2008;33:102–7.Google Scholar
- 29.Skopińska-Różewska E, Wasiutyński A, Sommer E, et al. The infuence of Rhodiola rosea, Rhodiola kirilowii and Rhodiola quadrifida extracts on cutaneous angiogenesis induced in mice after graftin of human kidney cancer tissue. Cent Eur J Immunol. 2008;33:185–9.Google Scholar
- 35.Thompson JF, Soong S, Balch S, et al. Prognostic significance of mitotic rate in localized primary cutaneous melanoma: an analysis of patients in the Multi-Institutional American Joint Committee on cancer melanoma staging database. J Clin Oncol. 2011;29:2199–205.CrossRefPubMedPubMedCentralGoogle Scholar