Extracellular vesicles from women with breast cancer promote an epithelial-mesenchymal transition-like process in mammary epithelial cells MCF10A
Extracellular vesicles (EVs) mediate many stages of tumor progression including angiogenesis, escape from immune surveillance, and extracellular matrix degradation. We studied whether EVs from plasma of women with breast cancer are able to induce an epithelial-mesenchymal transition (EMT) process in mammary epithelial cells MCF10A. Our findings demonstrate that EVs from plasma of breast cancer patients induce a downregulation of E-cadherin expression and an increase of vimentin and N-cadherin expression. Moreover, EVs induce migration and invasion, as well as an increase of NFκB-DNA binding activity and MMP-2 and MMP-9 secretions. In summary, our findings demonstrate, for the first time, that EVs from breast cancer patients induce an EMT-like process in human mammary non-tumorigenic epithelial cells MCF10A.
KeywordsExtracellular vesicles Breast cancer EMT MCF10A
We are grateful to Nora Ruiz for her technical assistance. This work was supported by a grant from ICyTDF (224/2012). O. G-H and E. R-U are supported by a Conacyt Predoctoral Training Grant. M-C. G-V. was supported by a Post-Doctoral fellowship from ICyTDF.
All studied participants signed informed consent, and the ethics committee of Hospital Juarez de Mexico approved the protocol (ARP/CEI/HJM/137/2013).
Conflict of interest