Tumor Biology

, Volume 36, Issue 12, pp 9649–9659 | Cite as

Extracellular vesicles from women with breast cancer promote an epithelial-mesenchymal transition-like process in mammary epithelial cells MCF10A

  • Octavio Galindo-Hernandez
  • Cristina Gonzales-Vazquez
  • Pedro Cortes-Reynosa
  • Emmanuel Reyes-Uribe
  • Sonia Chavez-Ocaña
  • Octavio Reyes-Hernandez
  • Mónica Sierra-Martinez
  • Eduardo Perez Salazar
Research Article

Abstract

Extracellular vesicles (EVs) mediate many stages of tumor progression including angiogenesis, escape from immune surveillance, and extracellular matrix degradation. We studied whether EVs from plasma of women with breast cancer are able to induce an epithelial-mesenchymal transition (EMT) process in mammary epithelial cells MCF10A. Our findings demonstrate that EVs from plasma of breast cancer patients induce a downregulation of E-cadherin expression and an increase of vimentin and N-cadherin expression. Moreover, EVs induce migration and invasion, as well as an increase of NFκB-DNA binding activity and MMP-2 and MMP-9 secretions. In summary, our findings demonstrate, for the first time, that EVs from breast cancer patients induce an EMT-like process in human mammary non-tumorigenic epithelial cells MCF10A.

Keywords

Extracellular vesicles Breast cancer EMT MCF10A 

Notes

Acknowledgments

We are grateful to Nora Ruiz for her technical assistance. This work was supported by a grant from ICyTDF (224/2012). O. G-H and E. R-U are supported by a Conacyt Predoctoral Training Grant. M-C. G-V. was supported by a Post-Doctoral fellowship from ICyTDF.

Ethical standards

All studied participants signed informed consent, and the ethics committee of Hospital Juarez de Mexico approved the protocol (ARP/CEI/HJM/137/2013).

Conflict of interest

None.

Supplementary material

13277_2015_3711_MOESM1_ESM.tif (245 kb)
Figure S1 EVs of breast cancer patients induce migration in MCF10A cells Cultures of MCF10A cells were scracth-wounded and treated with EV fractions from breast cancer patients Stage IIA (n = 5); Stage IIB (n = 4) and Stage IV (n = 1) and healthy women (n = 7). Pictures were taken at 48 h after wounding and are shown representative results. The graph represents the mean ± S.D. and is expressed as fold migration above control value (healthy women). (TIFF 245 kb)
13277_2015_3711_Fig6_ESM.gif (4 kb)

High resolution image (GIF 3 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Octavio Galindo-Hernandez
    • 1
  • Cristina Gonzales-Vazquez
    • 1
  • Pedro Cortes-Reynosa
    • 1
  • Emmanuel Reyes-Uribe
    • 1
  • Sonia Chavez-Ocaña
    • 2
  • Octavio Reyes-Hernandez
    • 2
  • Mónica Sierra-Martinez
    • 2
  • Eduardo Perez Salazar
    • 1
  1. 1.Departamento de Biologia CelularCinvestav-IPNMexicoMexico
  2. 2.Laboratorio de Genetica y Diagnostico MolecularUnidad de Investigacion, Hospital Juarez de MexicoMexicoMexico

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