Tumor Biology

, Volume 36, Issue 12, pp 9517–9524 | Cite as

Carbonic anhydrase IX overexpression regulates the migration and progression in oral squamous cell carcinoma

  • Jia-Sin Yang
  • Chiao-Wen Lin
  • Chun-Yi Chuang
  • Shih-Chi Su
  • Shu-Hui Lin
  • Shun-Fa Yang
Research Article


Carbonic anhydrase IX (CAIX) is reportedly overexpressed in several types of carcinomas and is generally considered a marker of malignancy. The current study investigated the association between membrane expression of CAIX and the clinicopathological characteristics in oral squamous cell carcinoma (OSCC) patients. The study used immunohistochemistry to examine CAIX expression in 271 OSCC specimens by tissue microarray (TMA) and assessed the effect of CAIX overexpression and knockdown on migration of oral cancer cells in vitro. We found that CAIX expression was associated with more advanced clinical stages (p = 0.030) and positive lymph node metastasis (p = 0.026). Importantly, CAIX expression was correlated with a poorer patient prognosis in a univariate survival analysis (p = 0.025). Moreover, CAIX suppression by small interfering RNA (siRNA) significantly reduced cellular migration in OECM-1 oral cancer cell. In conclusion, our study showed that the expression of CAIX in OSCC samples can predict the progression of OSCC and survival of OSCC patients in Taiwan.


CAIX Migration Tissue microarray Prognosis OSCC 



This study was supported by a research grant from the National Science Council, Taiwan (NSC96-2628-B-040-021-MY3 and NSC102-2314-B-040-008-MY3), and Chung Shan Medical University Hospital, Taiwan (CSH-2015-C-015; CSH-2015-E-001-Y2). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Jia-Sin Yang
    • 1
    • 2
  • Chiao-Wen Lin
    • 3
    • 4
  • Chun-Yi Chuang
    • 5
    • 6
  • Shih-Chi Su
    • 7
    • 8
  • Shu-Hui Lin
    • 2
    • 9
  • Shun-Fa Yang
    • 1
    • 2
  1. 1.Department of Medical ResearchChung Shan Medical University HospitalTaichungTaiwan
  2. 2.Institute of MedicineChung Shan Medical UniversityTaichung 402Taiwan
  3. 3.Institute of Oral SciencesChung Shan Medical UniversityTaichungTaiwan
  4. 4.Department of DentistryChung Shan Medical University HospitalTaichungTaiwan
  5. 5.School of MedicineChung Shan Medical UniversityTaichungTaiwan
  6. 6.Department of OtolaryngologyChung Shan Medical University HospitalTaichungTaiwan
  7. 7.Department of Dermatology, Drug Hypersensitivity Clinical and Research CenterChang Gung Memorial HospitalLinkouTaiwan
  8. 8.Whole-Genome Research Core Laboratory of Human DiseasesChang Gung Memorial HospitalKeelungTaiwan
  9. 9.Department of Surgical PathologyChanghua Christian HospitalChanghuaTaiwan

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