Tumor Biology

, Volume 36, Issue 12, pp 9511–9516 | Cite as

Promoter hypermethylation of the cysteine protease RECK may cause metastasis of osteosarcoma

  • Leisheng Wang
  • Junbo Ge
  • Tian Ma
  • Yanpin Zheng
  • Shiqiao Lv
  • Yu Li
  • Shaoxian Liu
Research Article


The present study examined the role of reversion-inducing cysteine-rich protein with Kazal motifs (RECK) promoter hypermethylation as a causative factor in metastasis of osteosarcoma. Using human pathological samples, it is demonstrated that RECK, a cysteine protease that reversibly regulates expression of matrix metalloproteases like matrix metallopeptidase 9 (MMP9), is transcriptionally inhibited in osteosarcoma, especially metastatic variants. This result comes from its promoter hypermethylation, as evaluated in the present study by methylation-specific PCR reaction. The expression of RECK was also significantly diminished in the metastatic variants of osteosarcoma. This downregulation of RECK in advanced grades of osteosarcoma and metastatic grades was also associated with the increased expression of invadosome-specific markers like MMP9, phospho-FAK, and integrins, suggesting the complex contributions of RECK in the prevention of metastasis and its downregulation as a causative factor in osteosarcoma metastasis.


RECK Promoter hypermethylation Metastasis Bone tumors Osteosarcoma 



Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Leisheng Wang
    • 1
    • 2
  • Junbo Ge
    • 2
  • Tian Ma
    • 3
  • Yanpin Zheng
    • 1
  • Shiqiao Lv
    • 2
  • Yu Li
    • 2
  • Shaoxian Liu
    • 2
  1. 1.Department of Orthopedics, Qihu HospitalShandong UniversityJinanChina
  2. 2.Department of OrthopedicsYantaishan HospitalYantaiChina
  3. 3.Department of OrthopedicsThe People’s Hospital of QixiaQixiaChina

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