Fuzzy clustering demonstrates that codon 72 SNP rs1042522 of TP53 gene associated with HNSCC but not with prognoses
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It is estimated that 7.6 million people will die as a consequence of head and neck squamous cell carcinoma (HNSCC). Genetic predisposition has emerged as an important risk factor in the development and prognosis of HNSCC. Considering this, the aim of the current study is to assess whether codon 72 SNP of the TP53 gene (rs1042522) is associated with an increased odds ratio of developing HNSCC or with a worse prognosis in patients with HNSCC. Analysis of the rs1042522 in HNSCC patients and in control individuals. Differences between the case and control groups were determined using chi-squared tests. Multivariate analysis was performed to evaluate the odds ratio of HNSCC. Fussy C Means Clustering was to cluster HNSCC patients for survival analyses. Time of survival was calculated using the Kaplan-Meier estimator and comparing this to the log rank test. Statistical significance was set at p < 0.05. A total of 71.4 % of the Arg/Arg genotype were from HNSCC patients, while only 28.6 % of Arg/Arg genotype were found in the control group. Logistic regression demonstrated that the Arg/Arg genotype, smoking, and alcohol consumption increase the odds ratio of HNSCC. No association between TP53 codon 72 polymorphism and P53 expression. No association between rs1042522 and survival or prognoses was observed. This study identified that individuals carrying the arginine allele at rs1042522 have an increased odds ratio of HNSCC. However, no association between codon 72 SNP of the TP53 gene and HNSCC prognosis or P53 expression was observed.
KeywordsTumor suppressor gene Survival Oral cancer Survival p53
This study was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and the Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). Dr.Guimarães, Dr. Santos, and Dr. de Paula are research fellows of the CNPq.
- 2.(INCA) NCI: Estimate 2014: Brazilian cancer incidence.: Instituto Nacional deCâncer. 2014.Google Scholar
- 5.Gritz ER, Carr CR, Rapkin DA, Chang C, Beumer J, Ward PH. A smoking cessation intervention for head and neck cancer patients: trial design, patient accrual, and characteristics. Cancer Epidemiol Biomark Prev. 1991;1:67–73.Google Scholar
- 9.Alves LR, Fraga CA, Oliveira MV, Sousa AA, Jorge AS, Marques-Silva L, et al. High hif-1alpha expression genotypes increase odds ratio of oral cancer. Head Neck Oncol. 2012;4:87.Google Scholar
- 10.Fraga CA, Sousa AA, Correa GT, Jorge AS, Jesus SF, Jones KM, et al. High hypoxia-inducible factor-1alpha expression genotype associated with eastern cooperative oncology group performance in head and neck squamous cell carcinoma. Head Neck Oncol. 2012;4:77.Google Scholar
- 15.Stefani FA, Viana MB, Dupim AC, Brito JA, Gomez RS, da Costa JE, et al. Expression, polymorphism and methylation pattern of interleukin-6 in periodontal tissues. Immunobiology. 2012;218:2012–7.Google Scholar
- 30.Correa GT, Bandeira GA, Cavalcanti BG, Santos FB, Neto JF, Guimaraes AL, et al. Analysis of ecog performance status in head and neck squamous cell carcinoma patients: association with sociodemographical and clinical factors, and overall survival. Support Care Cancer. 2012;20:2679–85.CrossRefPubMedGoogle Scholar
- 35.Ferreira da Silva I, Koifman RJ, Quinto Santos Souza C, Ferreira de Almeida Neto O, Koifman S. Tp53 genetic polymorphisms and environmental risk factors associated with cervical carcinogenesis in a cohort of brazilian women with cervical lesions. J Toxicol Environ Health A. 2010;73:888–900.CrossRefPubMedGoogle Scholar
- 40.Linder S, Parrado C, Falkmer UG, Blasjo M, Sundelin P, von Rosen A. Prognostic significance of ki-67 antigen and p53 protein expression in pancreatic duct carcinoma: a study of the monoclonal antibodies mib-1 and do-7 in formalin-fixed paraffin-embedded tumour material. Br J Cancer. 1997;76:54–9.CrossRefPubMedPubMedCentralGoogle Scholar
- 42.de Oliveira MV, Pereira Gomes EP, Pereira CS, de Souza LR, Barros LO, Mendes DC, et al. Prognostic value of microvessel density and p53 expression on the locoregional metastasis and survival of the patients with head and neck squamous cell carcinoma. Appl Immunohistochem Mol Morphol: AIMM / Off Publ Soc Appl Immunohistochem. 2013;21:444–51.CrossRefGoogle Scholar