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Fuzzy clustering demonstrates that codon 72 SNP rs1042522 of TP53 gene associated with HNSCC but not with prognoses

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Tumor Biology

Abstract

It is estimated that 7.6 million people will die as a consequence of head and neck squamous cell carcinoma (HNSCC). Genetic predisposition has emerged as an important risk factor in the development and prognosis of HNSCC. Considering this, the aim of the current study is to assess whether codon 72 SNP of the TP53 gene (rs1042522) is associated with an increased odds ratio of developing HNSCC or with a worse prognosis in patients with HNSCC. Analysis of the rs1042522 in HNSCC patients and in control individuals. Differences between the case and control groups were determined using chi-squared tests. Multivariate analysis was performed to evaluate the odds ratio of HNSCC. Fussy C Means Clustering was to cluster HNSCC patients for survival analyses. Time of survival was calculated using the Kaplan-Meier estimator and comparing this to the log rank test. Statistical significance was set at p < 0.05. A total of 71.4 % of the Arg/Arg genotype were from HNSCC patients, while only 28.6 % of Arg/Arg genotype were found in the control group. Logistic regression demonstrated that the Arg/Arg genotype, smoking, and alcohol consumption increase the odds ratio of HNSCC. No association between TP53 codon 72 polymorphism and P53 expression. No association between rs1042522 and survival or prognoses was observed. This study identified that individuals carrying the arginine allele at rs1042522 have an increased odds ratio of HNSCC. However, no association between codon 72 SNP of the TP53 gene and HNSCC prognosis or P53 expression was observed.

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Acknowledgments

This study was supported by grants from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), and the Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG). Dr.Guimarães, Dr. Santos, and Dr. de Paula are research fellows of the CNPq.

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Authors and Affiliations

  1. Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Brazil

    Ugo Borges Pinheiro, Carlos Alberto de Carvalho Fraga, Danilo Cangussu Mendes, Lucyana Conceição Farias, Alfredo Maurício Batista de Paula & André Luiz Sena Guimarães

  2. Department of Medicine and Department of Computer Science, Universidade Estadual de Montes Claros, Montes Claros, Brazil

    Cláudio Marcelo Cardoso & Christine Mendes Silveira

  3. Universidade Estadual de Montes Claros, Montes Claros, Brazil

    Marcos Flávio Silveira Vasconcelos D̕Angelo

  4. Center for Research, The Educative Association of Brazil, Montes Claros, Brazil

    Kimberly Marie Jones

  5. Department Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil

    Sérgio Henrique Souza Santos

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  1. Ugo Borges Pinheiro
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Correspondence to André Luiz Sena Guimarães.

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Supplementary Figure 3

Survival of HNSCC patients according to TNM. The results of the Kaplan-Meier estimator were compared to the log rank test. (A) was without Fuzzy C Means clustering (p = 0.03) and (B) was with Fuzzy C Means clustering (p = 0.02) (GIF 16 kb)

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Pinheiro, U.B., de Carvalho Fraga, C.A., Mendes, D.C. et al. Fuzzy clustering demonstrates that codon 72 SNP rs1042522 of TP53 gene associated with HNSCC but not with prognoses. Tumor Biol. 36, 9259–9265 (2015). https://doi.org/10.1007/s13277-015-3677-7

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