Tumor Biology

, Volume 36, Issue 12, pp 9365–9372 | Cite as

Targeting DNA-PKcs increased anticancer drug sensitivity by suppressing DNA damage repair in osteosarcoma cell line MG63

  • Xin Li
  • Jiguang Tian
  • Qiyu Bo
  • Ka Li
  • Hongliang Wang
  • Ting Liu
  • Jianmin Li
Research Article


Many chemotherapy drugs exert anticancer effects through causing DNA damage, such as DNA topoisomerase inhibitor and platinum-containing drugs. DNA damage repair is an important mechanism of drug resistance which is responsible for metastasis and recurrence after chemotherapy. DNA-dependent protein kinase (DNA-PK) plays an important role in non-homology end joining (NHEJ) pathway. In this study, we aimed to determine whether DNA-PK catalytic subunit (DNA-PKcs) is expressed in osteosarcoma MG63 cell line and involved in drug resistance induced by DNA repair. We found that DNA-PKcs was expressed in osteosarcoma cell line MG63. The pDNA-PKcsT2609 was more expressed in cells treated with cisplatin (DDP) and etoposide (VP16). Down-regulation of DNA-PKcs produced higher sensitivity of MG63 cells to DDP or VP16 through increasing apoptosis and causing cell cycle arrest in the G1 phase. Our study supported that DNA-PKcs was involved in drug-induced DNA damage repair and related to chemosensitivity of osteosarcoma MG63 cells.


DNA-PKcs Osteosarcoma DNA damage repair Drug resistance 



This study was funded by the National Natural Science Foundation of China (81172551) and the Shandong Technological Development Project (ZR2011HM037).

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Xin Li
    • 1
  • Jiguang Tian
    • 2
  • Qiyu Bo
    • 3
  • Ka Li
    • 1
  • Hongliang Wang
    • 1
  • Ting Liu
    • 4
  • Jianmin Li
    • 1
  1. 1.Department of Orthopedics, Qilu HospitalShandong UniversityShandongChina
  2. 2.Emergency Department, Qilu HospitalShandong UniversityShandongChina
  3. 3.Operating room of Qilu HospitalShandong UniversityShandongChina
  4. 4.Department of Obstetrics and Gynecology, Qilu HospitalShandong UniversityShandongChina

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