Tumor Biology

, Volume 36, Issue 11, pp 8747–8754 | Cite as

Aberrant expression of long noncoding RNAs in colorectal cancer with liver metastasis

  • Le-chi Ye
  • Li Ren
  • Jun-jun Qiu
  • De-xiang Zhu
  • Tao Chen
  • Wen-ju Chang
  • Shi-xu Lv
  • Jianmin Xu
Research Article


Long noncoding RNA (lncRNA) plays a crucial role in the regulation of various cellular processes and human diseases. However, little is known about the role of lncRNAs in colorectal liver metastasis (CLM). In the present study, we aimed to determine whether lncRNAs are differentially expressed in CLM tissue and to further assess their clinical value. lncRNA arrays were employed to screen for differentially expressed lncRNAs in colorectal cancer (CRC) tissues with synchronous, metachronous, or nonliver metastasis. Based on bioinformatics data, a quantitative reverse-transcription polymerase chain reaction (qRT-PCR) assay was performed to identify target lncRNAs in an expanded set of CRC samples with various subtypes of liver metastasis. The relationships between the target lncRNAs and the clinical characteristics and patient prognosis were further analyzed. After determining the expression profile of lncRNAs (n = 1332) in CLM tissue, 40 differentially expressed lncRNAs that were potentially related to CLM were selected for further examination in an expanded set of clinical samples, and three novel target lncRNAs, termed lncRNA-CLMAT1-3, were verified. High lncRNA-CLMAT3 expression strongly correlated with liver metastasis (P = 0.03) and lymph node metastasis (P = 0.009). Moreover, patients displaying high lncRNA-CLMAT3 expression exhibited a shorter median overall survival duration than those displaying low lncRNA-CLMAT3 expression (30.7 vs. 35.2 months, P = 0.007). Multivariate analysis demonstrated that the lncRNA-CLMAT3 expression level is an independent prognostic factor (hazard ratio 2.05, P = 0.02) after adjusting for other known prognostic factors. lncRNA-CLMAT3 over-expression was significantly associated with CLM and was an independent predictor of poor survival for patients with CRC.


Colorectal liver metastasis Long noncoding RNA lncRNA-CLMAT3 



We thank the National Natural Science Foundation of China (No. 81372315), Shanghai medical guide project (134119a4800) and Shanghai Scientific Research Plan Project (No. 13JC1401601) for providing funding supports.

Conflicts of interest


Authors’ contributions

Le-chi Ye and Li Ren performed the molecular genetics experiments, participated in the sequence alignment, and drafted the manuscript. Jun-jun Qiu participated in the sequence alignment. Tao Chen, De-xiang Zhu, and Wen-ju Chang participated in the design of the study and performed the statistical analysis. Jianmin Xu, Li Ren, and Ye Wei conceived of the study, participated in its design and coordination, and helped to draft the manuscript. All authors read and approved the final manuscript.

Supplementary material

13277_2015_3627_MOESM1_ESM.docx (48 kb)
ESM 1 (DOCX 47 kb)
13277_2015_3627_Fig4_ESM.jpg (185 kb)
Figure S1

Overview of the lncRNA microarray analysis results. Heat maps were generated based on hierarchical cluster analysis to display the differentially expressed lncRNAs (>2 fold-change in expression and P < 0.01) between the SLM and NCR samples (A), the MLM and NLM samples (B), the SLM and MLM samples (C), or the SLM and NLM samples (D). Abbreviations: CRC, colorectal cancer; NCR, normal colorectum; NLM, CRC with no liver metastasis; MLM, CRC with metachronous liver metastasis; SLM, CRC with synchronous liver metastasis. (JPEG 185 kb)

13277_2015_3627_Fig5_ESM.jpg (1.2 mb)
Figure S2

Validation of the microarray results. Five lncRNAs were randomly selected from the microarray data for validation via a qRT-PCR assay. (JPEG 1234 kb)

13277_2015_3627_Fig6_ESM.jpg (372 kb)
Figure S3

lncRNA-CLMAT3 (as indicated by the red arrow) neighbors the SPARC gene according to the data from the UCSC genome browser. (JPEG 372 kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Le-chi Ye
    • 1
    • 2
  • Li Ren
    • 2
  • Jun-jun Qiu
    • 3
  • De-xiang Zhu
    • 2
  • Tao Chen
    • 2
  • Wen-ju Chang
    • 2
  • Shi-xu Lv
    • 1
  • Jianmin Xu
    • 2
  1. 1.Department of Oncological SurgeryThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhouChina
  2. 2.Department of General Surgery, Zhongshan HospitalFudan UniversityShanghaiChina
  3. 3.Department of GynecologyObstetrics and Gynecology Hospital of Fudan UniversityShanghaiChina

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