Tumor Biology

, Volume 36, Issue 11, pp 8389–8398 | Cite as

miR-106a* inhibits the proliferation of renal carcinoma cells by targeting IRS-2

  • Yadong Ma
  • Hongyi Zhang
  • Xiaolong He
  • Hongxiong Song
  • Yayong Qiang
  • Yi Li
  • Jixue Gao
  • Ziming Wang
Research Article


MicroRNAs play critical roles in the development and progression of human cancers. Although it has been reported that miR-106a* is downregulated in follicular lymphoma, its role in renal cell carcinoma (RCC) remains unknown. This study investigated the expression and role of miR-106a* in human RCC. Our results showed that the miR-106a* expression decreased dramatically in clinical RCC tissues and cell lines. In vitro, overexpression of miR-106a* suppressed RCC cell proliferation and S/G2 transition, whereas inhibition of miR-106a* promoted cell proliferation and S/G2 transition. It was also found that miR-106a* expression was inversely correlated with the expression of insulin receptor substrate 2 (IRS-2). IRS-2 was determined to be a direct target of miR-106a* by a luciferase reporter assay. Importantly, silencing IRS-2 resulted in the same biologic effects as those of miR-106a* overexpression in RCC cells, including inhibition of RCC cell proliferation and triggering of S/G2 cell cycle arrest with inhibition of the PI3K/Akt signaling pathway. These results indicate that miR-106a* affects RCC progression by targeting IRS-2 with suppression of the PI3K/Akt signaling pathway in RCC cells. The findings suggest miR-106a* as a novel strategy for RCC treatment.


Renal cell carcinoma miR-106a* Proliferation Insulin receptor substrates 2 PI3K/Akt signaling pathway 



The authors would like to thank Professor Qi Chen for polishing the language in this manuscript.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yadong Ma
    • 1
    • 2
  • Hongyi Zhang
    • 2
  • Xiaolong He
    • 2
  • Hongxiong Song
    • 2
  • Yayong Qiang
    • 2
  • Yi Li
    • 2
  • Jixue Gao
    • 2
  • Ziming Wang
    • 1
  1. 1.Department of Urology, the Second Affiliated Hospital, School of MedicineXi’an Jiaotong UniversityXi’anChina
  2. 2.Department of Urology, Affiliated HospitalYan’an UniversityYan’an CityChina

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