miR-582-5p inhibits proliferation of hepatocellular carcinoma by targeting CDK1 and AKT3
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microRNAs play an important role in the progression of hepatocellular carcinoma (HCC). In this study, we found that miR-582-5p expression was downregulated in hepatoma tissues and HCC cell lines. Upregulation of miR-582-5p reduced colony number, inhibited cellular proliferation, and arrested cell cycle in G0/G1 phase. When miR-582-5p was inhibited, the colony number was increased and cellular proliferation and cell cycle were promoted. Further studies showed that miR-582-5p regulated the progression of HCC through directly inhibiting the expression of CDK1 and AKT3, and indirectly inhibiting the expression of cyclinD1.
KeywordsHepatocellular carcinoma miR-582-5p CDK1 AKT3 CyclinD1
The study was supported by The Natural Science Foundation of China, No: 30371394; Natural Science Fund of Hubei Province, No: 2012FFA044; the Health Department Found of Hubei Province, No: JX6B18; Public Service Platform Construction Projects of Wuhan Technology Bureau, No: 2013060705010326.
Conflicts of interest
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