The relationship between telomere length and clinicopathologic characteristics in colorectal cancers among Tunisian patients
- 149 Downloads
Alterations in telomere dynamics have emerged as having a causative role in carcinogenesis. Both the telomere attrition contribute to tumor initiation via increasing chromosomal instability and that the telomere elongation induces cell immortalization and leads to tumor progression. The objectives of this study are to investigate the dynamics of telomere length in colorectal cancer (CRC) and the clinicopathological parameters implicated. We measured the relative telomere length (RTL) in cancerous tissues and in corresponding peripheral blood leukocytes (PBL) using quantitative PCR (Q-PCR) from 94 patients with CRC. Telomere length correlated significantly in cancer tissues and corresponding PBL (r = 0.705). Overall, cancer tissue had shorter telomeres than PBL (p = 0.033). In both cancer tissue and PBL, the RTL was significantly correlated with age groups (p = 0.008 and p = 0.012, respectively). The RTL in cancer tissue was significantly longer in rectal tumors (p = 0.04) and in the late stage of tumors (p = 0.01). In PBL, the RTL was significantly correlated with the macroscopic aspect of tumors (p = 0.02). In addition, the telomere-length ratio of cancer to corresponding PBL increased significantly with late-stage groups. Shortening of the telomere was detected in 44.7 %, elongation in 36.2 %, and telomeres were unchanged in 19.1 % of 94 tumors. Telomere shortening occurred more frequently in the early stage of tumors (p = 0.01). This study suggests that the telomere length in PBL is affected by the macroscopic aspect of tumors and that telomere length in cancer tissues is a marker for progression of CRC and depends on tumor-origin site.
KeywordsTelomere length Colorectal cancer Clinicopathologic characteristics
We thank Dr. Neila Attia Romdhane for her help in the statistics part and Dr. Kawthar Ellouz for her english editing.
- 2.Lin KW. Telomeres, telomerase, and tumorigenesis a review. Medscape Gen Med. 2004;6(3).Google Scholar
- 6.Londono-Vallejo JA. Telomere instability and cancer. Biogeosciences. 2008;90:73–82.Google Scholar
- 7.De Cian A, Lacroix L, Douarre C, Temime-Smaali N, Trentesaux C, Riou JF, et al. Targeting telomeres and telomerase. Biogeosciences. 2008;90:131–55.Google Scholar
- 14.Shay JW. Determining if telomeres matter in colon cancer initiation or progression. J Natl Cancer Inst. 2013.Google Scholar
- 15.Svenson U, Roos G. Telomere length as a biological marker in malignancy. Biochim Biophys Acta. 2009;317:1792–4.Google Scholar
- 33.Ehrlenbach S, Willeit P, Kiechl S, Willeit J, Reindl M, Schanda K, Kronenberg F, Brandstätter A. Influences on the reduction of relative telomere length over 10 years in the population-based Bruneck Study: introduction of a well-controlled high-throughput assay. Int J Epidemiol. 2009;1–10.Google Scholar
- 34.Mather KA, Jorm AF, Parslow RA, Christensen H. Is telomere length a biomarker of aging? A review. J Gerontol A Biol Sci Med Sci. 2010.Google Scholar
- 39.Biray Avci C. Telomeres and lifestyle choices. Ege University Turkey. Reviews on Selected Topics of Telomere Biology. chapitre 7; p175-193.Google Scholar