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CMTM3 inhibits cell growth and migration and predicts favorable survival in oral squamous cell carcinoma

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Tumor Biology

Abstract

Downregulation of CKLF-like MARVEL transmembrane domain-containing member 3 (CMTM3) has been reported in a number of human tumors. However, the role of CMTM3 in oral squamous cell carcinoma (OSCC) remains largely unknown. In this study, we showed that the expression of CMTM3 was significantly reduced in OSCC cell lines and primary tumor specimens (P < 0.001). Methylation-specific PCR showed hypermethylation in CMTM3 promoter in a significant proportion of tumor tissues (61 %). The expression of CMTM3 was associated with T stage, lymph node metastasis, tumor node metastasis (TNM) stage, and recurrence of OSCC patients (P < 0.05, n = 201). More importantly, CMTM3 expression was associated with the prognosis of OSCC patients (P < 0.001) and was an independent prognostic factor (hazard ratio = 0.593, 95 % confidence interval, 0.272–1.292; P = 0.039). Overexpression of CMTM3 inhibited the growth and migration of OSCC cells. In vivo experiments also showed that the growth of OSCC xenografts in nude mice was significantly inhibited by CMTM3 overexpression. These findings indicate that downregulation of CMTM3 due to promoter hypermethylation contributed to the proliferation and migration of OSCC cells and suggest that CMTM3 is an independent prognostic factor for the evaluation of the survival of OSCC patients.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant numbers 81300894, 81030018, 30901680).

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Authors and Affiliations

  1. Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, China

    Heyu Zhang, Xuefen Li & Lisha Sun

  2. Department of Oral Pathology, Peking University School and Hospital of Stomatology, 22 South Zhongguancun Avenue, Haidian District, Beijing, 100081, China

    Jianyun Zhang, Jiafei Qu, Yingying Hong, Yan Chen & Tiejun Li

  3. Center for Human Disease Genomics, School of Basic Medical Sciences, Peking University, Beijing, 100191, China

    Xu Nan

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  1. Heyu Zhang
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  2. Jianyun Zhang
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  3. Xu Nan
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  4. Xuefen Li
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  9. Tiejun Li
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Correspondence to Tiejun Li.

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Zhang, H., Zhang, J., Nan, X. et al. CMTM3 inhibits cell growth and migration and predicts favorable survival in oral squamous cell carcinoma. Tumor Biol. 36, 7849–7858 (2015). https://doi.org/10.1007/s13277-015-3504-1

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  • DOI: https://doi.org/10.1007/s13277-015-3504-1

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