Inducing cell growth arrest and apoptosis by silencing long non-coding RNA PCAT-1 in human bladder cancer
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Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs that play important roles in cancer development and progression. Prostate cancer-associated transcript 1 (PCAT-1) is a novel lncRNA that promotes cell proliferation in prostate cancer. We hypothesized that PCAT-1 also have roles in bladder cancer. In this study, we found that PCAT-1 was up-regulated in bladder cancer compared to paired normal urothelium. Cell proliferation inhibition and apoptosis induction were also observed in PCAT-1 small hairpin RNA (shRNA)-transfected bladder cancer T24 and 5637 cells. Our data suggest that PCAT-1 plays oncogenic roles and can be used as a therapeutic target for treating human bladder cancer.
KeywordsPCAT-1 Bladder cancer Proliferation Apoptosis
The authors are indebted to the donors, whose names were not included in the author list, but who participated in this program. This work was funded by the National Key Basic Research Program of China (973 Program) (2014CB745201), National Natural Science Foundation of China (81402103, 81100460), Basic Research Program of Shenzhen (JCYJ20120614155650545), Shenzhen Foundation of Science and Technology (GJHZ20140414170821192, JCYJ20140414170821337), and “San Ming” Project for Medical Treatment and Public Health at Shenzhen.
Conflicts of interest