Tumor Biology

, Volume 36, Issue 10, pp 7685–7689 | Cite as

Inducing cell growth arrest and apoptosis by silencing long non-coding RNA PCAT-1 in human bladder cancer

  • Li Liu
  • Yuchen Liu
  • Chengle Zhuang
  • Wen Xu
  • Xing Fu
  • Zhaojie Lv
  • Hanwei Wu
  • Lisha Mou
  • Guoping Zhao
  • Zhiming Cai
  • Weiren Huang
Research Article

Abstract

Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs that play important roles in cancer development and progression. Prostate cancer-associated transcript 1 (PCAT-1) is a novel lncRNA that promotes cell proliferation in prostate cancer. We hypothesized that PCAT-1 also have roles in bladder cancer. In this study, we found that PCAT-1 was up-regulated in bladder cancer compared to paired normal urothelium. Cell proliferation inhibition and apoptosis induction were also observed in PCAT-1 small hairpin RNA (shRNA)-transfected bladder cancer T24 and 5637 cells. Our data suggest that PCAT-1 plays oncogenic roles and can be used as a therapeutic target for treating human bladder cancer.

Keywords

PCAT-1 Bladder cancer Proliferation Apoptosis 

Notes

Acknowledgments

The authors are indebted to the donors, whose names were not included in the author list, but who participated in this program. This work was funded by the National Key Basic Research Program of China (973 Program) (2014CB745201), National Natural Science Foundation of China (81402103, 81100460), Basic Research Program of Shenzhen (JCYJ20120614155650545), Shenzhen Foundation of Science and Technology (GJHZ20140414170821192, JCYJ20140414170821337), and “San Ming” Project for Medical Treatment and Public Health at Shenzhen.

Conflicts of interest

None

Supplementary material

13277_2015_3490_MOESM1_ESM.doc (64 kb)
ESM 1 (DOC 63 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Li Liu
    • 1
    • 2
  • Yuchen Liu
    • 1
  • Chengle Zhuang
    • 1
    • 2
  • Wen Xu
    • 3
  • Xing Fu
    • 1
  • Zhaojie Lv
    • 1
  • Hanwei Wu
    • 1
  • Lisha Mou
    • 1
  • Guoping Zhao
    • 1
    • 4
  • Zhiming Cai
    • 1
    • 2
  • Weiren Huang
    • 1
  1. 1.Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People’s HospitalThe First Affiliated Hospital of Shenzhen UniversityShenzhenChina
  2. 2.Shantou University Medical CollegeShantouChina
  3. 3.State Key Laboratory Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life SciencesSun Yat-sen UniversityGuangzhouChina
  4. 4.Shanghai-MOST Key Laboratory of Health and Disease GenomicsChinese National Human Genome Center at ShanghaiShanghaiChina

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