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Tumor Biology

, Volume 36, Issue 10, pp 7641–7647 | Cite as

RETRACTED ARTICLE: MiR-497 promotes metastasis of colorectal cancer cells through Nrdp1 inhibition

  • Yongsheng Jiang
  • Qinghua Meng
  • Jiaqin Qi
  • Haiyu Shen
  • Shaochuan Sun
Research Article

Abstract

We have recently shown that Nrdp1 inhibits phosphorylation of ErB3 in colorectal cancer (CRC) cells, to suppress epidermal growth factor receptor (EGFR) signaling-stimulated MMP7 activation for CRC metastasis. In this study, we examined the control of Nrdp1 in CRC cells. We detected significant increases in miR-497 in CRC specimen, compared to paired normal colorectal tissue. Moreover, we detected a strong positive correlation between miR-497 levels and Nrdp1 levels, and a strong inverse correlation between miR-497 levels and MMP7 levels. In vitro, overexpression of miR-497 in human CRC cells significantly decreased Nrdp1 transcripts and protein, and vice versa. Moreover, overexpression of miR-497 in human CRC cells also significantly increased MMP7 transcripts, cellular protein, and secreted protein, resulting in increases in cell invasiveness in a transwell cell migration assay. Furthermore, we found that MiR-497 directly targeted 3′-UTR of Nrdp1 mRNA to inhibit its translation. Together, our data suggest that the regulation of MMP7 by Nrdp1 in CRC cells could be inhibited by miR-497 through suppressing Nrdp1 translation. Our work thus highlights a novel molecular regulatory machinery that regulates metastasis of CRC.

Keywords

Neuregulin receptor degradation protein-1 (Nrdp1) Colorectal cancer (CRC) MMP7 miR-497 

Notes

Conflict of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yongsheng Jiang
    • 1
  • Qinghua Meng
    • 1
  • Jiaqin Qi
    • 1
  • Haiyu Shen
    • 1
  • Shaochuan Sun
    • 1
  1. 1.Department of General SurgeryJinan Central Hospital of Shandong UniversityJinanChina

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