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Tumor Biology

, Volume 37, Issue 1, pp 1289–1297 | Cite as

Elevated expression of FoxM1 promotes the tumor cell proliferation in hepatocellular carcinoma

  • Min Yu
  • Zheng Tang
  • Fandi Meng
  • Minghui Tai
  • Jingyao Zhang
  • Ruitao Wang
  • Chang Liu
  • Qifei Wu
Research Article

Abstract

The Forkhead box M1 (FoxM1) transcription factor plays crucial roles in multiple biological processes, including cell proliferation, differentiation, migration, and transformation. Recent studies have reported that aberrant expression of FoxM1 was found in a variety of human cancers. However, the expression pattern of FoxM1 and its clinical significance in human hepatocellular carcinoma (HCC) have not been well characterized to date. In this study, the expression of FoxM1 was evaluated in 46 pairs of human HCC, the adjacent non-tumorous liver tissues, and 12 pairs of normal liver tissues by immumohistochemistry. FoxM1 expression was upregulated in the HCC (76.09 %) compared with non-tumorous liver tissues (39.13 %) and normal liver tissues (8.33 %) (P < 0.05). FoxM1 expression was significantly associated with tumor stage, tumor size, tumor number, integrality of tumor encapsulation, tumor thrombus, and AFP level (P < 0.05). Functionally, enforced expression of FoxM1 in HCC cell line (HHCC) remarkably enhanced cell proliferation in vitro and in vivo. Further analysis of cell cycle-related molecules showed that FoxM1 overexpression increased expressions of cyclin B1 and cyclin D1 but reduced expressions of p27Kip1 and p21Cip1. Our findings suggest that FoxM1 overexpression promotes HCC cell proliferation by cell cycle regulation, which is a potential target for hepatocellular carcinoma therapy.

Keywords

Cell cycle Forkhead box M1 Cell proliferation Hepatocellular carcinoma 

Notes

Acknowledgments

This project was supported by National Natural Science Foundation of China (30901447/C160402), China Postdoctoral Science Foundation funded project (2013M532058), and Key Science and Technology Project of Shaanxi Province (2013K12-08-01).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anPeople’s Republic of China
  2. 2.Department of Hepatopancreatobiliary SurgeryThe Central Hospital of Jinhua CityJinhuaChina
  3. 3.Department of Forensic MedicineXinxiang Medical CollegeXinxiangChina
  4. 4.Department of Thoracic Surgery, the First Affiliated Hospital of Xi’an Jiaotong UniversityXi’anPeople’s Republic of China

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