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Tumor Biology

, Volume 36, Issue 12, pp 9327–9337 | Cite as

The expression of microRNA-34a is inversely correlated with c-MET and CDK6 and has a prognostic significance in lung adenocarcinoma patients

  • Ji Hyung Hong
  • Kang San Roh
  • Sung-Suk Suh
  • Sukchan Lee
  • Sook Whan Sung
  • Jae Kil Park
  • Jae Ho Byun
  • Jin Hyoung Kang
Research Article

Abstract

We aimed to establish whether the expression of microRNA-34a (miR-34a) is correlated with that of c-MET and G1 phase regulators such as cyclin dependent kinase (CDK) 4, CDK6, and cyclin D (CCND) 1 in non-small cell lung cancer (NSCLC), and whether a relationship exists between miR-34a expression and both clinicopathologic factors and recurrence-free survival (RFS). For 58 samples archived from NSCLC patients, we measured the expression of miR-34a and c-MET, CDK4/6, and CCND1 by quantitative RT-PCR and assessed the relationship between miR-34a expression, clinicopathological factors, and RFS. The expression of miR-34a was significantly lower in squamous cell tumors (P < 0.001) and in tumors associated with lymphatic invasion (P = 0.001). We found significant inverse correlations between miR-34a and c-MET (R = −0.316, P = 0.028) and CDK6 expression (R = −0.4582, P = 0.004). RFS were longer in adenocarcinoma patients with high miR-34a expression than in those with low miR-34a expression (55.6 vs. 21.6 months; P = 0.020). With univariate analysis, statistically significant prognostic factors for RFS in adenocarcinoma patients were miR-34a expression (Relative risk (RR), 8.14; P = 0.049), TNM stage (RR, 13.55; P = 0.001), LN metastasis (RR, 4.19; P = 0.043), and the presence of lymphatic invasion (RR, 7.05; P = 0.015). In multivariate analysis, only miR-34a was prognostic for RFS (RR, 11.5; P = 0.027). miR-34a expression was inversely correlated with that of c-MET and CDK6 in NSCLC, and had prognostic significance for RFS, especially in adenocarcinoma patients.

Keywords

miR-34a c-MET Cyclin-dependent kinase 6 Adenocarcinoma of the lung 

Notes

Acknowledgments

None.

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Ji Hyung Hong
    • 1
  • Kang San Roh
    • 2
  • Sung-Suk Suh
    • 3
  • Sukchan Lee
    • 2
  • Sook Whan Sung
    • 4
  • Jae Kil Park
    • 4
  • Jae Ho Byun
    • 1
  • Jin Hyoung Kang
    • 1
  1. 1.Department of Internal Medicine, College of Medicine, Seoul St. Mary’s HospitalThe Catholic University of KoreaSeoulRepublic of Korea
  2. 2.Department of Genetic EngineeringSungkyunkwan UniversitySuwonRepublic of Korea
  3. 3.South Sea Environment Research DepartmentKorea Institute of Ocean Science and TechnologyGeojeRepublic of Korea
  4. 4.Department of Thoracic Surgery, College of MedicineThe Catholic University of KoreaSeoulRepublic of Korea

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