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Tumor Biology

, Volume 36, Issue 9, pp 7085–7091 | Cite as

A Aconitum coreanum polysaccharide fraction induces apoptosis of hepatocellular carcinoma (HCC) cells via pituitary tumor transforming gene 1 (PTTG1)-mediated suppression of the P13K/Akt and activation of p38 MAPK signaling pathway and displays antitumor activity in vivo

  • Ming Liang
  • Jianchao Liu
  • Hongyu Ji
  • Moyang Chen
  • Yonghua Zhao
  • Shuchen Li
  • Xiaoyu Zhang
  • Jingyuan Li
Research Article

Abstract

In this study, we observed that a Aconitum coreanum polysaccharide (CACP) exhibited an effective inhibitory effect on H22 cell growth in vitro and in vivo via the induction of apoptosis. Further, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting assays revealed that the expression of pituitary tumor transforming gene 1 (PTTG1), one proto-oncogene, was evidently suppressed in both transcript and protein levels in H22 cell model or mice after CACP treatment. Particularly, CACP (40 μg/ml) treatment or transfection with PTTG1 small interfering RNA (siRNA) could greatly reduce the phosphorylation of Akt (p-Akt) but increase phospho-p38 mitogen-activated protein kinase (p-p38 MARK) protein levels in H22 cells as compared with vehicle-treated cells. Likewise, following treatment of H22-tumor-bearing mice with CACP (100 mg/kg), doxorubicin (DOX, 3 mg/kg), and their combination, tumor tissues showed an attenuated p-Akt protein expression, but a striking p-p38 MARK level when compared with those in model mice. Taken together, we demonstrated here the inhibitory effect of CACP on the growth of H22 cells in vitro and in vivo, which may be through, at least partly, repression of PTTG1 and then followed by the inactivation of P13/Akt and activation of p38 MARK signaling pathways. These findings offered a novel approach for the treatment of hepatocellular carcinoma (HCC) in the future.

Keywords

Aconitum coreanum Polysaccharides Antitumor Pituitary tumor transforming gene 1 (PTTG1) P13K/Akt p38 MAPK 

Notes

Acknowledgment

This research was financed by the National Natural Science Foundation of China (No. 81303265).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Ming Liang
    • 1
  • Jianchao Liu
    • 1
  • Hongyu Ji
    • 1
  • Moyang Chen
    • 1
  • Yonghua Zhao
    • 1
  • Shuchen Li
    • 1
  • Xiaoyu Zhang
    • 1
  • Jingyuan Li
    • 1
  1. 1.Department of Infectious DiseasesThe Second Affiliated Hospital of Harbin Medical UniversityHarbinChina

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