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Tumor Biology

, Volume 36, Issue 9, pp 6789–6795 | Cite as

Ginsenoside Rh2 inhibits metastasis of glioblastoma multiforme through Akt-regulated MMP13

  • Ning Guan
  • Xiaochuan Huo
  • Zhenxing Zhang
  • Shoudan Zhang
  • Junsheng Luo
  • Wenshi Guo
Research Article

Abstract

Glioblastoma multiforme (GBM) is the most malignant type of primary brain tumor. Although the growth of the tumor cells in a relatively closed space may partially account for its malignancy, highly invasive nature of glioblastoma cells has been suggested to be the main reason for the failure of current therapeutic approaches. Ginsenoside Rh2 (GRh2) has recently been shown to significantly suppress the growth and survival of GBM through inhibiting epidermal growth factor receptor signaling, whereas its effects on the invasion and metastasis have not been examined. Here, we showed that GRh2 dose-dependently decreased GBM cell invasiveness in both scratch wound healing assay and Transwell cell migration assay. Moreover, the inhibitory effects of GRh2 on cell migration seemed to be conducted through decreased expression of matrix metalloproteinase (MMP)-13. Furthermore, using specific inhibitors, we found that GRh2 inhibited MMP13 through PI3k/Akt signaling pathway. Finally, high MMP13 levels were detected in GBM specimen from the patients. Together, these data suggest that GRh2 may suppress GBM migration through inhibiting Akt-mediated MMP13 activation. Thus, our data highlight a previous unappreciated role for GRh2 in suppressing GBM cell metastasis.

Keywords

Glioblastoma multiforme (GBM) Ginsenoside Rh2 (GRh2) PI3k Akt Matrix metalloproteinase (MMP)-13 

Notes

Acknowledgments

This work was supported by Non-profit foundation of Liaoning Province Technology Department, No.: 2013001009 and Aohongboze Foundation of Liaoning Medical College, No.: XZJJ20130206.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Ning Guan
    • 1
  • Xiaochuan Huo
    • 1
  • Zhenxing Zhang
    • 1
  • Shoudan Zhang
    • 1
  • Junsheng Luo
    • 1
  • Wenshi Guo
    • 1
  1. 1.Department of NeurosurgeryThe First Affiliated Hospital of Liaoning Medical UniversityJinzhouChina

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