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Tumor Biology

, Volume 36, Issue 9, pp 6883–6889 | Cite as

Decreased MT1-MMP in gastric cancer suppressed cell migration and invasion via regulating MMPs and EMT

  • Wenfeng Li
  • Shouzhi Li
  • Liang Deng
  • Shibin Yang
  • Mingzhe Li
  • Shuo Long
  • Sile Chen
  • Fuxiang Lin
  • Longbin Xiao
Research Article

Abstract

Membrane type 1-matrix metalloproteinase (MT1-MMP) has been identified to play a significant role in several types of cancers, but little is known about the significance of MT1-MMP in gastric cancer patients. The purpose of this study is to investigate the involvement of MT1-MMP in tumor progression of gastric cancer. MT1-MMP expression levels were examined in gastric cancer tissues and cells, and normal gastric tissues and cells. The effects and molecular mechanisms of MT1-MMP expression on cell proliferation, migration, and invasion were also explored. In our results, MT1-MMP messenger RNA (mRNA) and protein expression levels were significantly increased in gastric cancer tissue. Moreover, the overexpression of MT1-MMP was positively associated with the status of clinical stage and lymph node metastasis through real-time PCR. Furthermore, knocking down MT1-MMP expression significantly suppressed the cell migration and invasion in vitro and regulated the expression of MMPs and epithelial-mesenchymal transition (EMT)-associated genes. In conclusions, our study demonstrates that MT1-MMP was overexpressed in gastric cancer tissue, and reduced expression of MT1-MMP suppressed cell migration, invasion, and through regulating the expression of MMPs and the process of EMT in gastric cancer.

Keywords

MT1-MMP Gastric cancer Biomarker Metastasis Epithelial-mesenchymal transition 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Wenfeng Li
    • 1
  • Shouzhi Li
    • 1
  • Liang Deng
    • 1
  • Shibin Yang
    • 1
  • Mingzhe Li
    • 1
  • Shuo Long
    • 1
  • Sile Chen
    • 1
  • Fuxiang Lin
    • 1
  • Longbin Xiao
    • 1
  1. 1.Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina

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