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Decreased expression of RPS15A suppresses proliferation of lung cancer cells

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Tumor Biology

Abstract

Lung cancer is the leading cause of cancer-related death in the world. Previous report has identified ribosomal protein s15a (RPS15A) as a TGF-β-responsible gene in the lung adenocarcinoma cell line A549. In this study, we used specific si-RNA to downregulate RPS15A expression in A549 cells and found that decreased RPS15A expression significantly inhibited cell proliferation and survival, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays. Moreover, A549 cells were obviously accumulated in the G0/G1 phase in response to RPS15A knockdown, suggesting that RPS15A inhibition could induce a diminution of proliferation through cell cycle arrest. In addition, immunohistochemistry analysis further revealed that RPS15A was overexpressed in surgically resected lung cancer tissues. In conclusion, we identify RPS15A as a novel potential oncogenic gene involved in lung carcinogenesis. This study may provide a preliminary experimental basis for a gene therapy approach for treating lung cancer.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (no. 81401892).

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Authors and Affiliations

  1. Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China

    Xinmin Zhao, Yu Feng, Hui Yu, Xianghua Wu, Jianhua Chang, Jie Qiao & Jialei Wang

  2. Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China

    Xinmin Zhao, Lei Shen, Yu Feng, Hui Yu, Xianghua Wu, Jianhua Chang, Xuxia Shen, Jie Qiao & Jialei Wang

  3. Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China

    Lei Shen & Xuxia Shen

Authors
  1. Xinmin Zhao
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  2. Lei Shen
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  3. Yu Feng
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  4. Hui Yu
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  5. Xianghua Wu
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  6. Jianhua Chang
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  7. Xuxia Shen
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  8. Jie Qiao
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  9. Jialei Wang
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Correspondence to Jialei Wang.

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Zhao, X., Shen, L., Feng, Y. et al. Decreased expression of RPS15A suppresses proliferation of lung cancer cells. Tumor Biol. 36, 6733–6740 (2015). https://doi.org/10.1007/s13277-015-3371-9

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  • DOI: https://doi.org/10.1007/s13277-015-3371-9

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