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MicroRNA-506 inhibits gastric cancer proliferation and invasion by directly targeting Yap1

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Tumor Biology

Abstract

Increasing evidence indicates that microRNA (miR)-506 plays a vital role in tumorigenesis; however, the role of miR-506 in gastric cancer (GC) is unclear and needs further investigation. In the present study, we showed that the decrease in the expression of miR-506 is associated with tumor size, pathological tumor node metastasis (TNM) stage, and lymph node metastasis in 63 GC patient tumors. We found that patients with lower expression of miR-506 had a poor prognosis than that with the patients with high expression of miR-506. Notably, the ectopic expression of miR-506 was sufficient to inhibit cell proliferation, invasion, and epithelial-mesenchymal transition in the GC cells. Moreover, results from luciferase reporter assays identified miR-506 as a direct regulator of Yes-associated protein 1 (Yap1). Reintroduction of Yap1 rescues miR-506-induced effects on SGC-7901 cell proliferation and invasion. This function of miR-506/Yap1 axis is clinically significant, as the level of miR-506 is inversely correlated with Yap1 mRNA expression in matched tissues. Thus, our study demonstrates that miR-506 may act as a tumor suppressor in GC and that the miR-506/Yap1 axis may help us better understand the molecular mechanisms of GC progression.

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Acknowledgments

This work was supported by the JiangXi Province Talent 555 Project and the National Natural Science Foundation of China (No. 81160281 and 81441083).

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Authors and Affiliations

  1. Department of Oncology, The First Affiliated Hospital of Nanchang University, Nanchang, 330006, China

    Jun Deng, Wan Lei, Xiaojun Xiang, Ling Zhang, Feng Yu, Jun Chen, Miao Feng & Jianping Xiong

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  1. Jun Deng
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  2. Wan Lei
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  3. Xiaojun Xiang
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  4. Ling Zhang
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  5. Feng Yu
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  6. Jun Chen
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  7. Miao Feng
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  8. Jianping Xiong
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Correspondence to Jianping Xiong.

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Jun Deng is the first author.

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Deng, J., Lei, W., Xiang, X. et al. MicroRNA-506 inhibits gastric cancer proliferation and invasion by directly targeting Yap1. Tumor Biol. 36, 6823–6831 (2015). https://doi.org/10.1007/s13277-015-3364-8

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  • DOI: https://doi.org/10.1007/s13277-015-3364-8

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