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Tumor Biology

, Volume 36, Issue 9, pp 6781–6788 | Cite as

A polysaccharide from the alkaline extract of Glycyrrhiza inflata induces apoptosis of human oral cancer SCC-25 cells via mitochondrial pathway

  • Guang Zeng
  • Huan Shen
  • Guo Tang
  • Xingwei Cai
  • Lixia Bi
  • Bin Sun
  • Yongjin Yang
  • Wenxing Xun
Research Article

Abstract

In the present study, we isolated and characterized a homogenous polysaccharide (GIAP1) from the alkaline extract of the roots of Glycyrrhiza inflata. The anti-tumor activity of GIAP1 toward human oral cancer SCC-25 cells and the underlying mechanisms were also examined in vitro. GIAP1 dose-dependently inhibited the proliferation of SCC-25 cells via inducing apoptosis. Moreover, GIAP1 downregulated Bax/Bcl-2 ratio, disrupted the mitochondrial membrane potential (MMP), and caused the release of cytochrome c to cytosol. Besides, GIAP1 triggered activation of capase-3 and caspase-9, as well as the degradation of poly (ADP-ribose) polymerase (PARP). In addition, the caspase-3 or caspase-9 inhibitor significantly inhibited GIAP1-induced apoptosis in SCC-25 cells. Collectively, we can conclude that the GIAP1 induces apoptosis in SCC-25 cells via a mitochondrial pathway.

Keywords

Glycyrrhiza inflata Polysaccharide Structure Apoptosis Human oral cancer Mitochondria 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Guang Zeng
    • 1
  • Huan Shen
    • 2
  • Guo Tang
    • 3
  • Xingwei Cai
    • 2
  • Lixia Bi
    • 2
  • Bin Sun
    • 1
  • Yongjin Yang
    • 2
  • Wenxing Xun
    • 1
  1. 1.Department of Plastic and Burn SurgeryTangdu Hospital, The Fourth Military Medical UniversityXi’anChina
  2. 2.Department of StomatologyThe General Hospital of the Second Artillery Corps of Chinese PLABeijingChina
  3. 3.Department of DermatologyThe First Affiliated Hospital of the General Hospital of the People’s Liberation ArmyBeijingChina

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