Tumor Biology

, Volume 36, Issue 8, pp 6285–6293 | Cite as

A Huaier polysaccharide reduced metastasis of human hepatocellular carcinoma SMMC-7721 cells via modulating AUF-1 signaling pathway

  • Cong Li
  • Xia Wu
  • Honghai Zhang
  • Gengxia Yang
  • Meijun Hao
  • Shoupeng Sheng
  • Yu Sun
  • Jiang Long
  • Caixia Hu
  • Xicai Sun
  • Li Li
  • Jiasheng Zheng
Research Article

Abstract

TP-1 is a polysaccharide from one famous fungus Huaier. Treatment with TP-1 significantly inhibited the cell growth, adhesion, migration, and motility of SMMC-7721 cells in a dose-dependent manner. Real-time quantitative RT-PCR revealed a dose-dependent decrease in RNA-binding factor 1 (AUF-1) and astrocyte elevated gene-1 (AEG-1) messenger RNA (mRNA) levels in TP-1-treated SMMC-7721 cells, which is consistent with their protein expression detected by Western blotting. On the contrary, microRNA-122 (miR-122) expression increased in SMMC-7721 cells following TP-1 treatment. Moreover, TP-1 treatment at three doses apparently increased epithelial marker E-cadherin protein expression but decreased the mesenchymal marker N-cadherin protein level. In addition, the hematoxylin-eosin (H & E) staining showed that the TP-1 significantly inhibited the lung metastasis of liver cancer in mice orthotopic implanted with SMMC-7721 tumor tissue. Taken together, these findings proved the inhibitory effect of TP-1 on the growth and metastasis of SMMC-7721 cells, and TP-1 might be offered for future application as a powerful chemopreventive agent against hepatocellular carcinoma (HCC) metastasis.

Keywords

Huaier polysaccharide Metastasis Hepatocellular carcinoma RNA-binding factor 1 (AUF-1) Astrocyte elevated gene-1 (AEG-1) Epithelial-mesenchymal transition (EMT) 

Notes

Acknowledgments

This research is funded by the National Natural Science Foundation of China (grant no. 81472328); Liver Disease AIDS Foundation of You An Hospital, Capital Medical University (BJYAH-2011-034); National Science and Technology Support Project (2012BAI15B08); and Key Project of National Communicable Disease (2012ZX10002015-002).

Conflicts of interest

The authors declare that they have no conflicts of interest concerning this article.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Cong Li
    • 1
  • Xia Wu
    • 2
  • Honghai Zhang
    • 1
  • Gengxia Yang
    • 1
  • Meijun Hao
    • 1
  • Shoupeng Sheng
    • 1
  • Yu Sun
    • 1
  • Jiang Long
    • 1
  • Caixia Hu
    • 1
  • Xicai Sun
    • 3
  • Li Li
    • 4
  • Jiasheng Zheng
    • 1
  1. 1.Intervention Therapy Center of Liver Diseases, Beijing You An HospitalCapital Medical UniversityBeijingChina
  2. 2.Department of Infectious Diseasethe Second Affiliated Hospital of Harbin Medical UniversityHarbinChina
  3. 3.School of Medicine, Tsinghua Center for Life SciencesTsinghua UniversityBeijingChina
  4. 4.Institute of Liver Diseases, Beijing You An HospitalCapital Medical UniversityBeijingChina

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