Tumor Biology

, Volume 36, Issue 8, pp 6265–6276 | Cite as

NFKB1/NFKBIa polymorphisms are associated with the progression of cervical carcinoma in HPV-infected postmenopausal women from rural area

  • Singhal Pallavi
  • Kumar Anoop
  • Hussain Showket
  • Nag Alo
  • Bharadwaj Mausumi
Research Article

Abstract

Human papillomavirus (HPV) is considered as the major etiological agent for development of cervical cancer but alone is not sufficient enough. So, other environmental factors and host genetic background may play an important role in the development of cervical cancer. HPV carries a minimal amount of structural and regulatory proteins so it is apparently dependent on its host for survival. NF-κB/IkB system plays an important regulatory role in the apoptotic pathway. In the present study, a total of 575 consecutive subjects including 285 cases (45 cervical pre-cancerous and 240 invasive cervical carcinoma) and 290 age- and ethnicity-matched controls recruited from Lok Nayak Jai Prakash Hospital and Safdarjung Hospital, New Delhi between July 2009 to July 2013 were genotyped for NFKB1 -94 insertion/deletion (rs28362491) and NFKBIa 3′-UTR2758A > G (rs696) polymorphism by PCR-RFLP followed by sequencing. We observed a positive association of NFKB1 -94 insertion/insertion (II) and NFKBIa 3′-UTR 2758 GG genotypes with the progression of cervical carcinoma. Cervical cancer patients were found more pronounce to be a carrier of II + GG genotype of both the SNPs. We also noticed that HPV-infected postmenopausal women having higher parity along with the history of tobacco consumption and who carries insertion allele of NFKB1 -94 polymorphism in association of GG genotype of NFKBIa 3′-UTR polymorphism, were more susceptible to develop cervical carcinoma. II + GG genotype together were found to have direct proportionality with the aggressiveness of cervical carcinoma. In conclusion, alteration in the gene map of NFKB1/NFKBIa helps in the progression of cervical cancer accompanied by HPV infection in postmenopausal women from rural residential setup who had higher parity along with history of tobacco consumption.

Keywords

NFKB1 NFKBIa Polymorphism HPV Cervical cancer 

Notes

Acknowledgments

We like to thank Dr. Sudha Salhan, Department of Obstetrics & Gynaecology, Safdarjung Hospital, New Delhi, and Dr. Swaraj Batra, Department of Obstetrics & Gynaecology, LNJP Hospital, New Delhi, India for providing clinical samples and valuable feedback for the manuscript. Dr. Suresh Bhambhani, Division of Cytopathology for pathological analysis of the clinical sample. PS is grateful to the Indian Council of Medical Research (ICMR) for Senior Research Fellowship and AK is for University Grant Commission for his Senior Research Fellowship. Core Facility of ICPO (ICMR) to MB is acknowledged. We also like to thank the patients and their family members.

Conflicts of interest

None

Supplementary material

13277_2015_3312_MOESM1_ESM.doc (45 kb)
ESM 1 (DOC 45 kb)
13277_2015_3312_MOESM2_ESM.doc (38 kb)
ESM 2 (DOC 38 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Singhal Pallavi
    • 1
    • 2
  • Kumar Anoop
    • 1
  • Hussain Showket
    • 1
  • Nag Alo
    • 2
  • Bharadwaj Mausumi
    • 1
  1. 1.Division of Molecular Genetics and BiochemistryInstitute of Cytology and Preventive OncologyNoidaIndia
  2. 2.Department of BiochemistryUniversity of Delhi South campus (UDSC)New DelhiIndia

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