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Tumor Biology

, Volume 36, Issue 8, pp 5901–5911 | Cite as

Proliferative role of TRAF4 in breast cancer by upregulating PRMT5 nuclear expression

  • Fan Yang
  • Jian Wang
  • Hua-yan Ren
  • Juan Jin
  • Ai-lian Wang
  • Li-li Sun
  • Ke-xin Diao
  • En-hua Wang
  • Xiao-yi Mi
Research Article

Abstract

In this study, we examined protein arginine methyltransferase 5 (PRMT5) and tumor necrosis factor receptor-associated 4 (TRAF4) expression in breast cancer to find the interaction mechanism between the two. We examined TRAF4 and PRMT5 expression by immunohistochemistry and found that their expression is positively correlated in breast cancer. Besides, PRMT5 expression was significantly associated with histological type and tumor size (p < 0.05). PRMT5 nuclear expression was significantly associated with HER2 expression (p < 0.05). PRMT5 and TRAF4 were both overexpressed in breast cancer tissues and cells, and we found that PRMT5 binds to the zinc finger structures in TRAF4 by coimmunoprecipitation and Western blotting. We also tested the potential regulatory effect between TRAF4 and PRMT5. TRAF4 upregulated PRMT5 expression, which occurred predominantly in the nucleus, on which TRAF4 promotion of cell proliferation in breast cancer is mainly dependent. PRMT5 may play an important role in activation of the NF-κB signaling pathway.

Keywords

TRAF4 PRMT5 Breast cancer NF-κB Cell proliferation 

Abbreviations

TRAF4

Tumor necrosis factor receptor-associated factor 4

PRMT5

Protein arginine methyltransferases 5

Notes

Acknowledgments

We thank Dr. Bert W. O’Malley (Baylor College of Medicine) for kindly providing plasmid for Zn fingers domain deletion mutant (Flag-△Zn-TRAF4). This work was supported by a grant from the Educational Commission of Liaoning Province of China (L2014302) and the Doctoral Program of Higher Education, China (20112104110017).

Conflicts of interest

None.

Supplementary material

13277_2015_3262_MOESM1_ESM.pdf (127 kb)
Fig. S1 PRMT5 did not influence TRAF4 expression. Western blot analyses showing that MCF-7 cells expressing the PRMT5 plasmid did not influence TRAF4 expression obviously, consistent with the finding that PRMT5 depletion by siRNA in MDA-MB-231 cells (p > 0.05). (PDF 127 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Fan Yang
    • 1
  • Jian Wang
    • 1
  • Hua-yan Ren
    • 1
  • Juan Jin
    • 1
  • Ai-lian Wang
    • 1
  • Li-li Sun
    • 1
  • Ke-xin Diao
    • 1
  • En-hua Wang
    • 1
  • Xiao-yi Mi
    • 1
  1. 1.Department of Pathology, First Affiliated Hospital and College of Basic Medical SciencesChina Medical UniversityShenyangChina

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