Androgen receptor promotes esophageal cancer cell migration and proliferation via matrix metalloproteinase 2
Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies worldwide. Androgen receptor (AR) plays an important role in many kinds of cancers. However, the molecular mechanisms of AR in ESCC are poorly characterized. In the present study, Western blot analysis and real-time quantitative PCR were performed to identify differentially expressed AR in 40 ESCC tissue samples, which revealed that the messenger RNA (mRNA) and protein expression of AR is upregulated in the ESCC tissue samples. AR overexpression induced increases in ESCC cell invasion and proliferation in vitro. Silencing of AR inhibited the proliferation of KYSE450 cells which have a relatively high level of AR, and the invasion of KYSE450 cells was distinctly suppressed. Furthermore, AR knockdown led to substantial reductions in matrix metalloproteinase 2 (MMP2) and p-AKT levels in ESCC cell lines, but no significant change in AKT and MMP9 expression. These results suggest that AR is involved in tumor progression, and thus, AR could represent selective targets for the molecularly targeted treatments of ESCC.
KeywordEsophageal squamous cell carcinoma Androgen receptor Matrix metalloproteinases Small interfering RNA Proliferation Invasion Targeted therapy
The study was funded by the grant “Experimental Study of the Splenic Artery Targeted Removal of Activated T Cells to Inhibit Acute Rejection”, 2012FFB03401, longitudinal support project by the Science and Technology Department of Hubei Province.
Conflicts of interest
- 4.Reid BJ, Prevo LJ, Galipeau PC, Sanchez CA, Longton G, et al. Predictors of progression in Barrett’s esophagus II: baseline 17p (p53) loss of heterozygosity identifies a patient subset at increased risk for neoplastic progression. Am J Gastroenterol. 2001;96:2839–48.CrossRefPubMedPubMedCentralGoogle Scholar