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Tumor Biology

, Volume 36, Issue 8, pp 5859–5864 | Cite as

Androgen receptor promotes esophageal cancer cell migration and proliferation via matrix metalloproteinase 2

  • Yi Zhang
  • Tiecheng Pan
  • Xiaoxuan Zhong
  • Cai Cheng
Research Article

Abstract

Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies worldwide. Androgen receptor (AR) plays an important role in many kinds of cancers. However, the molecular mechanisms of AR in ESCC are poorly characterized. In the present study, Western blot analysis and real-time quantitative PCR were performed to identify differentially expressed AR in 40 ESCC tissue samples, which revealed that the messenger RNA (mRNA) and protein expression of AR is upregulated in the ESCC tissue samples. AR overexpression induced increases in ESCC cell invasion and proliferation in vitro. Silencing of AR inhibited the proliferation of KYSE450 cells which have a relatively high level of AR, and the invasion of KYSE450 cells was distinctly suppressed. Furthermore, AR knockdown led to substantial reductions in matrix metalloproteinase 2 (MMP2) and p-AKT levels in ESCC cell lines, but no significant change in AKT and MMP9 expression. These results suggest that AR is involved in tumor progression, and thus, AR could represent selective targets for the molecularly targeted treatments of ESCC.

Keyword

Esophageal squamous cell carcinoma Androgen receptor Matrix metalloproteinases Small interfering RNA Proliferation Invasion Targeted therapy 

Notes

Acknowledgments

The study was funded by the grant “Experimental Study of the Splenic Artery Targeted Removal of Activated T Cells to Inhibit Acute Rejection”, 2012FFB03401, longitudinal support project by the Science and Technology Department of Hubei Province.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yi Zhang
    • 1
  • Tiecheng Pan
    • 2
  • Xiaoxuan Zhong
    • 1
    • 2
  • Cai Cheng
    • 2
  1. 1.Department of SurgeryThe Third Affiliated Hospital of Jianghan UniversityWuhanChina
  2. 2.Division of Cardiothoracic Surgery, Tongji Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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