Tumor Biology

, Volume 36, Issue 7, pp 5551–5559 | Cite as

Dormancy activation mechanism of oral cavity cancer stem cells

  • Xiang Chen
  • Xin Li
  • Baohong Zhao
  • Dehao Shang
  • Ming Zhong
  • Chunfu Deng
  • Xinshan Jia
Research Article

Abstract

Radiotherapy and chemotherapy are targeted primarily at rapidly proliferating cancer cells and are unable to eliminate cancer stem cells in the G0 phase. Thus, these treatments cannot prevent the recurrence and metastasis of cancer. Understanding the mechanisms by which cancer stem cells are maintained in the dormant G0 phase, and how they become active is key to developing new cancer therapies. The current study found that the anti-cancer drug 5-fluorouracil, acting on the oral squamous cell carcinoma KB cell line, selectively killed proliferating cells while sparing cells in the G0 phase. Bisulfite sequencing PCR showed that demethylation of the Sox2 promoter led to the expression of Sox2. This then resulted in the transformation of cancer stem cells from the G0 phase to the division stage and suggested that the transformation of cancer stem cells from the G0 phase to the division stage is closely related to an epigenetic modification of the cell.

Keywords

Oral cancer Cancer stem cells Dormancy Activation Mechanism 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant no. 81370100 to Xinshan Jia).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Xiang Chen
    • 1
  • Xin Li
    • 2
  • Baohong Zhao
    • 1
  • Dehao Shang
    • 1
  • Ming Zhong
    • 3
  • Chunfu Deng
    • 1
  • Xinshan Jia
    • 4
    • 5
  1. 1.Center of Implant Dentistry, School of StomatologyChina Medical UniversityShenyangChina
  2. 2.Department of Pharmacology, College of Life Science and BiopharmaceuticsShenyang Pharmaceutical UniversityShenyangChina
  3. 3.Department of Pathology, School of StomatologyChina Medical UniversityShenyangChina
  4. 4.Department of Pathology, the First Affiliated HospitalChina Medical UniversityShenyangChina
  5. 5.Department of Pathology, College of Basic Medical SciencesChina Medical UniversityShenyangChina

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