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Tumor Biology

, Volume 36, Issue 7, pp 5149–5156 | Cite as

Correlation between TGF-β1 gene 29 T > C single nucleotide polymorphism and clinicopathological characteristics of osteosarcoma

  • Yang Wu
  • Jinmin Zhao
  • Maolin He
Research Article

Abstract

Transforming growth factor (TGF)-β1 is the most abundant growth factor in human bone. Several polymorphisms have been described in the TGF-β1 gene. To explore the correlation between TGF-β1 gene single nucleotide polymorphism and the clinicopathological characteristics of osteosarcoma. TaqMAN PCR technique was used to detect the TGF-β1 gene polymorphism of 124 patients with osteosarcoma from last follow-up and 136 healthy controls. The difference of gender, age, and allele frequency between patient group and control group with χ 2 text were tested. The relationship between single nucleotide polymorphism and the risk of osteosarcoma with logistic regression and different survival rates of different genotypic patients with osteosarcoma through Kaplan-Meier were analyzed. There is no remarkable difference of the three genotypes in TGF-β1 gene 509C > T locus between the patient group and control group (P = 0.26). However, there are significant distributive differences in 29 T > C genotype (P = 0.04), which shows that patients carrying TT genotype have more risk to get osteosarcoma than patients carrying CC genotype (odds ratio (OR) = 2.10, 95 % confidence interval (CI) = 1.08–4.05). The percentage of T allele frequency of patient group, as 60.1 %, is larger than the control group, as 48.9 %. By comparing with patients carrying CC genotype, patients carrying TT genotype have two times risk of metastasis (OR = 2.30, 95 % CI = 1.05–5.06), and most of them are in the period of Enneking IIB (OR = 2.54, 95 % CI = 1.18–5.51). The survival analysis indicates that there is no any significant decrease when there is recurrence in patients carrying TT genotype. The morbidity and metastasis of osteosarcoma are relevant to TGF-β1 gene 29 T > C single nucleotide polymorphism.

Keywords

Osteosarcoma Single nucleotide polymorphism TGF-β1 Clinicopathological characteristics 

Notes

Acknowledgments

This research was supported by the Guangxi Innovative Program of Graduate Education (Grant No. YCSZ2013035).

Conflict of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of Orthopedic Trauma and Hand SurgeryThe First Affiliated Hospital of Guangxi Medical UniversityNanningChina
  2. 2.Division of Spinal Surgery, Research Center for Regenerative MedicineThe First Affiliated Hospital of Guangxi Medical UniversityNanningChina
  3. 3.Department of Orthopedic Trauma and Hand Surgery, Research Center for Regenerative MedicineThe First Affiliated Hospital of Guangxi Medical UniversityNanningChina

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