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EIF2C, Dicer, and Drosha are up-regulated along tumor progression and associated with poor prognosis in bladder carcinoma

Tumor Biology volume 36, pages 5071–5079 (2015)Cite this article

Abstract

EIF2C, Dicer, and Drosha are microRNA-regulating machinery components, which participate in microRNA intracellular process and transfer. Our research demonstrated the expression and clinical role of the microRNA-regulating machinery in bladder cancer. EIF2C1, EIF2C2, Dicer, and Drosha mRNA and protein levels were analyzed in 100 bladder carcinomas and 50 normal bladder tissues using quantitative polymerase chain reaction and Western blotting. EIF2C2, Dicer, and Drosha mRNAs and proteins were overexpressed in carcinoma compared with normal tissues, whereas EIF2C1 mRNA and protein were not obviously different. Moreover, immunohistochemistry was used to detect the expressions of EIF2C2, Dicer, and Drosha in 100 bladder carcinomas. There were higher EIF2C2, Dicer, and Drosha expressions in carcinomas than in the adjacent normal tissues, positive correlations being noted with clinical stage, histopathologic grade, and recurrence. Higher EIF2C2, Dicer, and Drosha expressions were related to shorter cancer-specific survival and shorter recurrence-free survival. Multivariate Cox analysis showed that EIF2C2 was an important risk factor in bladder cancer. In conclusion, EIF2C2, Dicer, and Drosha are more highly expressed in bladder carcinoma, promote the development of bladder cancer, and suggested a poor prognosis. Their clinical role in bladder carcinoma merits further research.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 81202000), Liaoning Provincial Natural Science Foundation (Grant No. 2013021066), and Shenyang City Project of Key Laboratory (Grant No. F13-293-1-00).

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Author information

Affiliations

  1. Department of Urology, The First Hospital of China Medical University, 155 Nanjing North Street, Heping District, Shenyang City, Liaoning Province, 110001, China

    Zhe Zhang, Chuize Kong, Jianbin Bi, Daxin Gong, Xiuyue Yu, Du Shi & Bo Zhan

  2. Department of Hematology, Shengjing Hospital of China Medical University, Shenyang City, 110022, China

    Guojun Zhang

  3. Department of Stress Response Science, Hirosaki University Graduate School of Medicine, Hirosaki, 036-8562, Japan

    Peng Ye

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  1. Zhe Zhang
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  2. Guojun Zhang
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  3. Chuize Kong
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  4. Jianbin Bi
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  5. Daxin Gong
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  6. Xiuyue Yu
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  7. Du Shi
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  9. Peng Ye
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Correspondence to Zhe Zhang.

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Zhang, Z., Zhang, G., Kong, C. et al. EIF2C, Dicer, and Drosha are up-regulated along tumor progression and associated with poor prognosis in bladder carcinoma. Tumor Biol. 36, 5071–5079 (2015). https://doi.org/10.1007/s13277-015-3158-z

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  • DOI: https://doi.org/10.1007/s13277-015-3158-z

Keywords

  • EIF2C1
  • EIF2C2
  • Dicer
  • Drosha
  • Bladder cancer
  • Clinicopathologic feature
  • Prognosis