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miR-342-3p targets RAP2B to suppress proliferation and invasion of non-small cell lung cancer cells

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Tumor Biology

Abstract

MicroRNAs (miRNAs) play critical roles in cancer development and progression. In this study, we examined the roles and molecular mechanisms of miR-342-3p in human non-small cell lung cancer (NSCLC). The results showed that miR-342-3p is downregulated in NSCLC cell lines and tissues, and its overexpression induces significant inhibition of NSCLC cell proliferation, invasion, and tumor growth in nude mice. In addition, miR-342-3p repressed RAP2B expression through interactions with its 3′-UTR region. Restoration of RAP2B expression reversed miR-342-3p-mediated inhibitory activity in NSCLC cells. Finally, analyses of miR-342-3p and RAP2B levels in NSCLCs revealed that miR-342-3p inversely correlated with RAP2B mRNA expression. Our collective findings provide preliminary evidence that miR-342-3p acts as a tumor suppressor in NSCLC through repression of RAP2B.

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Acknowledgment

This study was supported by two grant from the National Natural Science Foundation of China (No. 81071924 and 81372520).

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    Authors and Affiliations

    1. Department of Cardio-thoracic Surgery, Xinhua Hospital, School of Medicine, Shanghai JiaoTong University, Shanghai, 200092, China

      Xiao Xie, Mingsong Wang, Fangbao Ding, Haibo Xiao, Fengqing Hu, Rui Hu & Ju Mei

    2. Department of Cardio-thoracic Surgery, The First affiliated hospital of Soochow University, Suzhou, 215006, China

      Hongtao Liu

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    1. Xiao Xie
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    Correspondence to Ju Mei.

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    Xiao Xie and Hongtao Liu contributed equally to this work.

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    Xie, X., Liu, H., Wang, M. et al. miR-342-3p targets RAP2B to suppress proliferation and invasion of non-small cell lung cancer cells. Tumor Biol. 36, 5031–5038 (2015). https://doi.org/10.1007/s13277-015-3154-3

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    • DOI: https://doi.org/10.1007/s13277-015-3154-3

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