Tumor Biology

, Volume 36, Issue 7, pp 5021–5029 | Cite as

Long noncoding RNA aberrant expression profiles after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy of AGC ascertained by microarray analysis

  • Xiaoqi Zeng
  • Huijuan Shi
  • Jiping Wang
  • Shuzhong Cui
  • Hongsheng Tang
  • Xiangliang Zhang
Research Article

Abstract

Long noncoding RNAs (lncRNAs) have been shown to be involved in the development and progression of advanced gastric cancer (AGC). However, the roles of lncRNAs in advanced gastric cancer during the process of cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) are not well understood. A high-throughput microarray analysis was performed to compare the expression profiles of lncRNAs and messenger RNAs (mRNAs) in AGC serum samples during the process of CRS + HIPEC. Several potentially AGC-associated lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. Using abundant and varied probes, we were able to assess 33,045 lncRNAs and 30,215 mRNAs in our microarray. We found that 566 lncRNAs were differentially expressed (2-fold change) in AGC serum samples, indicating the significantly up- or downregulated lncRNAs play important roles in AGC during the process of CRS + HIPEC. Quantitative PCR results further verified that eight lncRNAs were aberrantly expressed in AGC serum samples after CRS + HIEC compared with matched serum sample before CRS + HIPEC. Among them, BC031243 and RP11-356I2.2 were the most aberrantly expressed lncRNAs, as estimated by quantitative PCR in six pairs of AGC serum samples. Our study demonstrated the expression patterns of lncRNAs in AGC serums before and after CRS + HIPEC by microarray. These results revealed that lncRNAs were differentially expressed during the process of CRS + HIPEC, suggesting that they might play key roles in tumor development.

Keywords

Advanced gastric cancer (AGC) Cytoreductive surgery (CRS) Hyperthermic intraperitoneal chemotherapy (HIPEC) LncRNAs 

Notes

Grant supports

This work was supported by Guangdong Province Natural Science Fund (No. S2013010016662), the health bureau of Guangdong province (Nos. A2014224 and B2014196), Science and Technology Planning Project of Guangdong Province (No. 2013B021800284), and the National Natural Science Foundation of China (Nos. 81201932 and 81372493).

Conflicts of interest

The authors declare no competing financial interests.

Author contributions

XQ. Z and HJ. S designed the research; XL. Z, JP. W, and HS. T, XQ. Z, HJ.S contributed equally to this work; other authors reviewed and revised the manuscript.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Xiaoqi Zeng
    • 1
  • Huijuan Shi
    • 2
  • Jiping Wang
    • 3
  • Shuzhong Cui
    • 1
  • Hongsheng Tang
    • 1
  • Xiangliang Zhang
    • 1
  1. 1.Department of Abdominal Surgery (Section 2)Affiliated Cancer Hospital of Guangzhou Medical UniversityGuangzhouChina
  2. 2.Department of PathologyThe First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina
  3. 3.Department of Surgery, Brigham and Women’s Hospital and Harvard Medical School; Departments of Surgery and PathologyMassachusetts General Hospital and Harvard Medical SchoolBostonUSA

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