Abstract
Lung cancer is the leading cause of cancer-related deaths worldwide that result from the combined effected of smoking exposure and genetic susceptibility. CHRNA3, a nicotinic acetylcholine receptor gene, was associated with lung cancer risk. The aim of this study was to identify whether CHRNA3 polymorphisms increase lung cancer risk directly or indirectly through smoking behavior in the Chinese Han individuals. We conducted a case–control study including 228 individuals with lung cancer and 301 healthy individuals. Seventeen known SNPs within CHRNA3 were selected for genotyping. Odds ratios (OR) and 95 % confidence interval (CI) were calculated by unconditional logistic regression with adjustment for gender and age. Two SNPs (rs8042059 and rs7177514) showed a 1.54-fold (p = 0.036; 95 % CI = 1.03–2.32) and 1.52-fold (p = 0.043; 95 % CI = 1.01–2.27) increased risk for lung cancer in smokers, respectively. Rs8042059 also showed a significant association for variant genotypes (CA/AA) compared with the wild-type genotype (CC), with an OR = 1.84 (p = 0.042; 95 % CI, 1.02–3.33) in the dominant model. In addition, the haplotype analysis found that the haplotypes “TCAC” and “CTGT,” composed of rs938682, rs12914385, rs11637630, and rs2869546, were associated with a 1.79-fold and 501-fold increased lung cancer risk, respectively. However, the polymorphisms of all SNPs were not significantly different between controls and cases among general or nonsmokers population. Rs8042059 and rs7177514 may increase lung cancer risk indirectly through smoking behavior in the Chinese Han population.
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Zheng R, Zeng H, Zhang S, Fan Y, Qiao Y, Zhou Q et al. Lung cancer incidence and mortality in China, 2010. Thoracic Cancer. 2014.
Subramanian J, Govindan R. Lung cancer in never smokers: a review. J Clin Oncol: Off J Am Soc Clin Oncol. 2007;25(5):561–70. doi:10.1200/jco.2006.06.8015.
Sun S, Schiller JH, Gazdar AF. Lung cancer in never smokers—a different disease. Nat Rev Cancer. 2007;7(10):778–90. doi:10.1038/nrc2190.
Landi MT, Chatterjee N, Yu K, Goldin LR, Goldstein AM, Rotunno M, et al. A genome-wide association study of lung cancer identifies a region of chromosome 5p15 associated with risk for adenocarcinoma. Am J Hum Genet. 2009;85(5):679–91.
Broderick P, Wang Y, Vijayakrishnan J, Matakidou A, Spitz MR, Eisen T, et al. Deciphering the impact of common genetic variation on lung cancer risk: a genome-wide association study. Cancer Res. 2009;69(16):6633–41.
Amos CI, Wu X, Broderick P, Gorlov IP, Gu J, Eisen T, et al. Genome-wide association scan of tag SNPs identifies a susceptibility locus for lung cancer at 15q25. 1. Nat Genet. 2008;40(5):616–22.
Liu P, Vikis HG, Wang D, Lu Y, Wang Y, Schwartz AG, et al. Familial aggregation of common sequence variants on 15q24-25.1 in lung cancer. J Natl Cancer Inst. 2008;100(18):1326–30.
Saccone SF, Hinrichs AL, Saccone NL, Chase GA, Konvicka K, Madden PA, et al. Cholinergic nicotinic receptor genes implicated in a nicotine dependence association study targeting 348 candidate genes with 3713 SNPs. Hum Mol Genet. 2007;16(1):36–49.
Minna JD. Nicotine exposure and bronchial epithelial cell nicotinic acetylcholine receptor expression in the pathogenesis of lung cancer. J Clin Investig. 2003;111(1):31–3.
Bierut L, Stitzel J, Wang J, Hinrichs A, Grucza R, Xuei X, et al. Variants in nicotinic receptors and risk for nicotine dependence. Am J Psychiatr. 2008;165(9):1163–71.
Schuller HM. Is cancer triggered by altered signalling of nicotinic acetylcholine receptors? Nat Rev Cancer. 2009;9(3):195–205.
Tsurutani J, Castillo SS, Brognard J, Granville CA, Zhang C, Gills JJ, et al. Tobacco components stimulate Akt-dependent proliferation and NFkappaB-dependent survival in lung cancer cells. Carcinogenesis. 2005;26(7):1182–95. doi:10.1093/carcin/bgi072.
Hung RJ, McKay JD, Gaborieau V, Boffetta P, Hashibe M, Zaridze D, et al. A susceptibility locus for lung cancer maps to nicotinic acetylcholine receptor subunit genes on 15q25. Nature. 2008;452(7187):633–7.
Ren JH, Jin M, He WS, Liu CW, Jiang S, Chen WH, Yang KY, Wu G, Zhang T: Association between chrna3 rs1051730 genotype and lung cancer risk in Chinese Han population: a case-control study. Journal of Huazhong University of Science and Technology Medical sciences = Hua zhong ke ji da xue xue bao Yi xue Ying De wen ban = Huazhong keji daxue xuebao Yixue Yingdewen ban 2013;33:897-901.
Sakoda LC, Loomis MM, Doherty JA, Neuhouser ML, Barnett MJ, Thornquist MD, et al. Chromosome 15q24-25.1 variants, diet, and lung cancer susceptibility in cigarette smokers. Cancer Causes Control. 2011;22(3):449–61.
Amos CI, Gorlov IP, Dong Q, Wu X, Zhang H, Lu EY, et al. nicotinic acetylcholine receptor region on chromosome 15q25 and lung cancer risk among African Americans: a case–control study. J Natl Cancer Inst. 2010;102(15)):1199–205.
Truong T, Hung RJ, Amos CI, Wu X, Bickeböller H, Rosenberger A, et al. Replication of lung cancer susceptibility loci at chromosomes 15q25, 5p15, and 6p21: a pooled analysis from the International Lung Cancer Consortium. J Natl Cancer Inst. 2010;102(13):959–71.
Gabriel S, Ziaugra L, Tabbaa D. SNP genotyping using the Sequenom MassARRAY iPLEX platform. Current protocols in human genetics. 2009:2.12. 1-2.. 6.
Thomas RK, Baker AC, DeBiasi RM, Winckler W, LaFramboise T, Lin WM, et al. High-throughput oncogene mutation profiling in human cancer. Nat Genet. 2007;39(3):347–51.
Adamec C. Example of the use of the nonparametric test. test X 2 for comparison of 2 independent examples. Cesk Zdrav. 1964;12:613–9.
Bland JM, Altman DG. Statistics notes: the odds ratio. BMJ Br Med J. 2000;320(7247):1468.
Solé X, Guinó E, Valls J, Iniesta R, Moreno V. SNPStats: a web tool for the analysis of association studies. Bioinformatics. 2006;22(15):1928–9.
Frusch N, Bosquee L, Louis R. Lung cancer. Epidemiology and etiologic factors. Rev Med Liege. 2007;62(9):548–53.
Hecht SS. Progress and challenges in selected areas of tobacco carcinogenesis. Chem Res Toxicol. 2008;21(1):160–71. doi:10.1021/tx7002068.
Improgo MR, Tapper AR, Gardner PD. Nicotinic acetylcholine receptor-mediated mechanisms in lung cancer. Biochem Pharmacol. 2011;82(8):1015–21.
Saccone NL, Wang JC, Breslau N, Johnson EO, Hatsukami D, Saccone SF, et al. The CHRNA5-CHRNA3-CHRNB4 nicotinic receptor subunit gene cluster affects risk for nicotine dependence in African-Americans and in European-Americans. Cancer Res. 2009;69(17):6848–56.
Weiss RB, Baker TB, Cannon DS, von Niederhausern A, Dunn DM, Matsunami N, et al. A candidate gene approach identifies the CHRNA5-A3-B4 region as a risk factor for age-dependent nicotine addiction. PLoS Genet. 2008;4(7):e1000125.
Russo P, Nastrucci C, Alzetta G, Szalai C. Tobacco habit: historical, cultural, neurobiological, and genetic features of people's relationship with an addictive drug. Perspect Biol Med. 2011;54(4):557–77.
Wang Y, Broderick P, Matakidou A, Eisen T, Houlston RS. Chromosome 15q25 (CHRNA3-CHRNA5) variation impacts indirectly on lung cancer risk. PLoS One. 2011;6(4):e19085.
Acknowledgments
This work is supported by the National 863 High-Technology Research and Development Program (No. 2012AA02A519) and National Science and Technology Major Project (No. 2012ZX09506001-007). We are grateful to all patients and individuals who participated in the study. We would also like to thank clinicians and other hospital staff who helped us to collect blood samples and data.
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Wenjing Zhou and Tingting Geng contributed equally to this work.
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Zhou, W., Geng, T., Wang, H. et al. CHRNA3 genetic polymorphism and the risk of lung cancer in the Chinese Han smoking population. Tumor Biol. 36, 4987–4992 (2015). https://doi.org/10.1007/s13277-015-3149-0
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DOI: https://doi.org/10.1007/s13277-015-3149-0