Tumor Biology

, Volume 36, Issue 6, pp 4851–4859 | Cite as

Decreased expression of long noncoding RNA MEG3 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer

Research Article

Abstract

Colorectal cancer (CRC) remains an important public health problem in the world. Long noncoding RNA (lncRNA) is an RNA molecular that is longer than 200 nucleotides and cannot be translated into a protein. Recent studies have shown that lncRNAs play important roles in carcinogenesis and cancer metastasis. The aim of this study was to evaluate the expression and biological role of lncRNA maternally expressed gene 3 (MEG3) in colorectal cancer. Quantitative real-time-PCR (qRT-PCR) was performed to investigate the expression of MEG3 in tumor tissues and corresponding nontumor colorectal tissues from 62 patients. The lower expression of MEG3 was remarkably correlated with low histological grade, deep tumor invasion, and advanced tumor node metastasis (TNM) stage. Multivariate analyses revealed that MEG3 expression served as an independent predictor for overall survival. Further experiments revealed that overexpressed MEG3 significantly inhibited CRC cell proliferation both in vitro and in vivo. In conclusion, our study demonstrated that MEG3 is involved in the development and progression of colorectal cancer by regulating cell proliferation and shows that MEG3 may be a potential diagnostic and prognostic target in patients with colorectal cancer.

Keywords

Long noncoding RNA MEG3 Colorectal cancer Cell proliferation 

Notes

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Cancer Research and Therapy CenterThe Second Affiliated Hospital of Southeast UniversityNanjingChina
  2. 2.Department of Biochemistry and Molecular BiologyNanjing Medical UniversityNanjingChina
  3. 3.Laboratory centerSubei People’s HospitalYangzhouChina
  4. 4.Departments of PathologyFirst Affiliated Hospital of Nanjing Medical UniversityNanjingChina
  5. 5.Department of OncologyFirst Affiliated Hospital of Nanjing Medical UniversityNanjingChina

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