Baicalein induces human osteosarcoma cell line MG-63 apoptosis via ROS-induced BNIP3 expression
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Baicalein, a flavonoid compound, is one of the active constituents of the root of Scutellariae Radix. Its antitumor effects have attracted widespread attention worldwide. One of its major functions is to induce the apoptosis of tumor cells, but the antitumor mechanism is currently unclear. In the present study, we found that baicalein increased MG-63 cell mortality in a dose-dependent manner. Meanwhile, baicalein activated apoptosis through induced intracellular reactive oxygen species (ROS) generation, and that ROS scavenger N-acetyl-cysteine (NAC), glutathione (GSH), and superoxide dismutase (SOD) apparently inhibited intracellular ROS production, consequently attenuating the baicalein-induced apoptosis. Baicalein also induce the mitochondrial fragmentation which precedes the cell apoptosis. This morphological alteration is accompanied by an increase in the expression of the protein BNIP3 as well as Mul1 and Drp1. Furthermore, we show that the inhibition of BNIP3 expression can inhibit cell apoptosis by baicalein treatment. Taken together, our results bring the evidence of a mechanism that links apoptosis and ROS-induced BNIP3 expression in MG-63 cells with bacalein treatment and suggest that baicalein has a good potential as an anti-osteosarcoma drug.
KeywordsApoptosis Osteosarcoma Baicalein ROS BNIP3
Reactive oxygen species
BCL2/adenovirus E1B 19 kDa interacting protein 3
Conflicts of interest
Fangfan Ye drafted the article. Honghan Wang, Lusi Zhang, Yongyi Zou, and HaiLong Han were in charge in acquisition of data, analysis and interpretation of data, and revision of the article. Jia Huang took control in the conception and design.
- 10.J-Y CHUANG, Y-Y TSAI, S-C CHEN, T-J HSIEH, J-G CHUNG. Induction of G0/G1 arrest and apoptosis by 3-hydroxycinnamic acid in human cervix epithelial carcinoma (HeLa) cells. In Vivo. 2005;19:683–8.Google Scholar
- 13.Esmaeili MA, Farimani MM, Kiaei M. Anticancer effect of calycopterin via PI3K/Akt and MAPK signaling pathways, ROS-mediated pathway and mitochondrial dysfunction in hepatoblastoma cancer (HepG2) cells. Mol Cell Biochem. 2014;1–15.Google Scholar
- 18.Takahashi H, Chen MC, Pham H, Angst E, King JC, Park J, et al. Baicalein, a component of Scutellaria baicalensis induces apoptosis by Mcl-1 down-regulation in human pancreatic cancer cells. Biochimica et Biophysica Acta (BBA)-Molecular. Cell Res. 2011;1813:1465–74.Google Scholar
- 20.Zhang Y, Song L, Cai L, Wei R, Hu H, Jin W (2012) Effects of baicalein on apoptosis, cell cycle arrest, migration and invasion of osteosarcoma cells. Food Chem Toxicol.Google Scholar
- 23.Han Y, Chen JZ (2012) Oxidative stress induces mitochondrial DNA damage and cytotoxicity through independent mechanisms in human cancer cells. BioMed Res Int 2013Google Scholar