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Tumor Biology

, Volume 36, Issue 6, pp 4661–4670 | Cite as

Association of HPV with genetic and epigenetic alterations in colorectal adenocarcinoma from Indian population

  • Ruhina S Laskar
  • Fazlur R Talukdar
  • Javed H Choudhury
  • Seram Anil Singh
  • Sharbadeb Kundu
  • Bishal Dhar
  • Rosy Mondal
  • Sankar Kumar Ghosh
Research Article

Abstract

Several studies from developing countries have shown human papillomavirus to be associated with colorectal cancers, but the molecular characteristics of such cancers are poorly known. We studied the various genetic variations like microsatellite instability (MSI), oncogenic mutations and epigenetic deregulations like CpG island methylation in HPV associated and nonassociated colorectal cancer patients from Indian population. HPV DNA was detected by PCR using My09/My11 and Gp5+/Gp6+ consensus primers and typed using HPV16 and HPV18 specific primers. MSI was detected using BAT 25 and BAT 26 markers, and mutation of KRAS, TP53 and BRAF V600E were detected by direct sequencing. Methyl specific polymerase chain reaction (MSP) was used to determine promoter methylation of the classical CIMP panel markers (P16, hMLH1, MINT1, MINT2 and MINT31) and other tumour-related genes (DAPK, RASSF1, BRCA1 and GSTP1). HPV DNA was detected in 34/93 (36.5 %) colorectal tumour tissues, HPV 18 being the predominant high-risk type. MSI was detected in 7.5 % cases; KRAS codon 12, 13, BRAF V600E and TP53 mutations were detected in 36.5, 3.2 and 37.6 % of the cases, respectively. CIMP-high was observed in 44.08 % cases. HPV presence was not associated with age, stage or grade of tumours, MSI or mutations in KRAS, TP53 or BRAF genes. Higher methylation frequencies of all genes/loci under study except RASSF1, as well as significantly higher CIMP-high characteristics were observed in HPV positive tumours as compared to negative cases. HPV in association with genetic and epigenetic features might be a potent risk factor for colorectal cancer in Indian population.

Keywords

Colorectal cancer HPV Genetic alterations Epigenetics CIMP KRAS BRAF P53 MSI Northeast India 

Notes

Acknowledgments

We thank the Department of Biotechnology, Government of India, DBT, for providing infrastructural support (grant number BT/Med/NE-SFC/2009). Our sincere thanks go to the Cachar Cancer Hospital and Research, Silchar Medical College and Hospital, Assam, and Agartalata Government Medical College, Tripura, for providing samples and data.

Conflict of interest

The authors declare no conflict of interest.

Supplementary material

13277_2015_3114_MOESM1_ESM.pdf (33 kb)
ESM 1 (PDF 33 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Ruhina S Laskar
    • 1
  • Fazlur R Talukdar
    • 1
  • Javed H Choudhury
    • 1
  • Seram Anil Singh
    • 1
  • Sharbadeb Kundu
    • 1
  • Bishal Dhar
    • 1
  • Rosy Mondal
    • 1
  • Sankar Kumar Ghosh
    • 1
  1. 1.Molecular Medicine Laboratory, Department of BiotechnologyAssam UniversitySilcharIndia

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