A novel tumor suppressor gene in basal cell carcinoma: inhibition of growth factor-2
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In loss of heterozygosity (LOH) studies at the chromosome 4q22-35 region, it was shown that the amount of deletion was high in basal cell carcinoma (BCC). It has been proposed that genes located in this chromosomal region could be tumor suppressor genes in BCC. It has been thought that deletions in the ING2 gene located in the same region can play a role in the pathophysiology of BCC and that deletions occurring in this region may influence the level of ING2 expression in BCC. Tumoral and non-tumoral tissues from 75 patients with BCC (45 men and 30 women) were included to the study. Lesions were excised by a surgical margin of 0.5 cm. After excision, RNA was isolated from tumoral and non-tumoral tissue samples. ING2 messenger RNA (mRNA) expression level was determined in tumoral and non-tumoral tissues by the real-time polymerase chain reaction (RT-PCR). It was detected that ING2 mRNA expression level decreased in tumoral tissues when compared to non-tumoral tissues from BCC patients (p = 0.0001). It was found that expression levels of this gene were comparable among patients with primary, recurrent, or multiple BCC. It is thought that ING2 gene expression level could contribute to the development of BCC but not be associated with the stage and the prognosis of the tumor.
KeywordsBasal cell carcinomas Tumor suppressor genes ING gene family Skin cancer UV radiation
This study with TF.11.11 ID number was supported by Gaziantep University Scientific Research Project Coordination.
Conflicts of interest
- 2.Lu YP, Lou YR, Li XH, Xie JG, Brash D, Huang MT, et al. Stimulatory effect of oral administration of green tea or caffeine on ultraviolet light-induced increases in epidermal wild-type p53, p21(WAF1/CIP1), and apoptotic sunburn cells in SKH-1 mice. Cancer Res. 2000;60(17):4785–91.PubMedGoogle Scholar
- 19.Li XH, Kikuchi K, Zheng Y, Noguchi A, Takahashi H, Nishida T, et al. Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma. Oral Oncol. 2011;47(3):217–23. doi: 10.1016/j.oraloncology.2011.01.004.CrossRefPubMedGoogle Scholar
- 23.Coles AH, Marfella CG, Imbalzano AN, Steinman HA, Garlick DS, Gerstein RM, et al. p37Ing1b regulates B-cell proliferation and cooperates with p53 to suppress diffuse large B-cell lymphomagenesis. Cancer Res. 2008;68(21):8705–14. doi: 10.1158/0008-5472.CAN-08-0923.CrossRefPubMedPubMedCentralGoogle Scholar
- 32.Bolshakov S, Walker CM, Strom SS, Selvan MS, Clayman GL, El-Naggar A, et al. p53 mutations in human aggressive and nonaggressive basal and squamous cell carcinomas. Clin Cancer Res: Off J Am Assoc Cancer Res. 2003;9(1):228–34.Google Scholar
- 35.Liu J, Xu XF, Yang WJ. [The effects of Hedgehog-Gli 1 signaling pathway on proliferation and apoptosis of hepatic stellate cells]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi. Chin J Hepatol. 2009;17(1):33–7.Google Scholar