Tumor Biology

, Volume 36, Issue 6, pp 4575–4582 | Cite as

Associations of polymorphisms in microRNAs with female breast cancer risk in Chinese population

  • Bangshun He
  • Yuqin Pan
  • Yeqiong Xu
  • Qiwen Deng
  • Huling Sun
  • Tianyi Gao
  • Shukui Wang
Research Article


Polymorphisms in microRNA (miRNA) have been discussed to be associated with breast cancer risk; however, the conclusions were always inconsistent in different ethnicities. This case–control study enrolled 450 breast cancer cases, and 450 health controls was carried out to investigate the association between six polymorphisms in miRNAs and breast cancer risk. Sequenom MassARRAY was used to detect the polymorphisms in miRNAs, and the immunohistochemistry assay was applied to detect the expression of estrogen receptor (ER) and progesterone receptor (PR) in cancer tissue. The data showed that the 3746444 GG was associated with increased breast cancer risk (adjusted odds ratio (OR) = 1.71, 95 % confidence interval (CI) = 1.16–2.52) and that rs2292832 CC (adjusted OR = 0.54, 95 % CI = 0.34–0.85) was associated with decreased breast cancer risk. In addition, menopausal status subgroup analysis revealed that rs3746444 GG (adjusted OR = 2.34, 95 % CI = 1.31–4.15) and GA/GG (adjusted OR = 1.60, 95 % CI = 1.08–2.37) genotypes were associated with increased breast cancer risk for the subgroup of women with premenopausal status, respectively. Moreover, rs2910164 GG (adjusted OR = 1.84, 95 % CI = 1.07–3.15) and CG/GG (adjusted OR = 1.47, 95 % CI = 1.01–2.15) genotypes were associated with increased breast cancer risk in the postmenopausal status subcohort, respectively. Furthermore, rs3746444 AG (adjusted OR = 1.61, 95 % CI = 1.06–2.45) and AG/GG (adjusted OR = 1.49, 95 % CI = 1.02–2.18) genotypes were observed to be associated with increased risk of lymph node involvement and breast cancer with negative PR expression, separately. In short, rs3746444 was associated with breast cancer risk, especially for women with premenopausal status, and rs2910164 CG and CG/GG genotypes were associated with breast cancer risk for the women with premenopausal status.


Breast cancer Polymorphism MicroRNA 



This study was supported by grants from the National Nature Science Foundation of China (no. 81200401) and Nanjing Medical Science and Technique Development Foundation to BS. H (nos. JQX13003, QRX11254, QYK11175) and YQ. P (no. QRX11255).

Conflicts of interest


Supplementary material

13277_2015_3102_MOESM1_ESM.docx (31 kb)
Supplementary table (DOCX 31 kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Bangshun He
    • 1
  • Yuqin Pan
    • 1
  • Yeqiong Xu
    • 2
  • Qiwen Deng
    • 1
  • Huling Sun
    • 3
  • Tianyi Gao
    • 1
  • Shukui Wang
    • 1
  1. 1.Central Laboratory, Nanjing First HospitalNanjing Medical UniversityNanjingChina
  2. 2.Changshu Medical Examination InstituteChangshuChina
  3. 3.Department of Life SciencesNanjing Normal UniversityNanjingChina

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