MicroRNA-10b and minichromosome maintenance complex component 5 gene as prognostic biomarkers in breast cancer
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The aim of this study is to identify micro-ribonucleic acid (microRNA) and its target, in addition to their relationship to the outcome in breast cancer (BC). To achieve this aim, we investigated microRNA-10b (miR-10b) and minichromosome maintenance complex component 5 (MCM5 mRNA) expression in 230 breast tissue samples by real-time PCR and semiquantitative conventional RT-PCR, respectively. Relapse-free survival (RFS) associated with miRNA-10b and MCM5 mRNA were tested by Kaplan–Meier survival analysis. The impact of miRNA-10b andMCM5 mRNA expression on the survival was evaluated by Cox proportional hazard regression model. The expression of miRNA-10b and MCM5 mRNA was positive in 86.4 and 79.7 % breast cancer patients, respectively. The overall concordance rate between miRNA-10b and MCM5 RNA was 90.4 %. The median follow-up period was 50 months. The survival analysis showed that high levels of both miR-10b and MCM5 were associated with short relapse free survival of BC. We identified MCM5 mRNA expression changes consistent with the miRNA-10b target regulation. Thus, we could consider miRNA-10b and MCM5 mRNA as prognostic markers and potential therapeutic targets in breast cancer to be applied to other patient data sets.
KeywordsmiRNA-10b Prognostic biomarker Breast cancer MCM5 Database Bioinformatics
Minichromosome maintenance complex component 5
Body mass index
- Her-2 neu
Human epidermal growth factor receptor 2
Invasive duct carcinoma
Invasive lobular carcinoma
Oral contraceptive therapy
This work was supported by Ain Shams University Research Projects 2014-15. Authors are grateful to Professor Fateen Anous, professor of surgery, Ain Shams University, for kindly providing surgical samples and patients data.
Conflicts of interest
- 1.Cava C, Bertoli G, Ripamonti M, Mauri G, Zoppis I, Della Rosa PA, et al. Integration of mRNA expression profile, copy number alterations, and microRNA expression levels in breast cancer to improve grade definition. PLoS One. 2014;9(5):e97681. doi: 10.1371/journal.pone.0097681. eCollection 2014.CrossRefPubMedPubMedCentralGoogle Scholar
- 4.Zhang JJ, Zhao Y, Chait BT, Lathem WW, Ritzi M, Knippers R, Darnell JE. Ser727-dependent recruitment of MCM5 by Stat1alpha in IFN-gamma-induced transcriptional activation. EMBO J. 1998.Google Scholar
- 8.Pinder SE, Elston CW, Ellis IO. Invasive carcinoma: usual histological types. In: Elston CW, Ellis IO, editors. The Breast. 3rd ed. Edinburgh: Churchill Livingstone; 1998. p. 283–337.Google Scholar
- 9.Sobin L, Gospodarowicz M, Wittekind C, editors. TNM classification of malignant tumors. 7th ed. Hoboken: John Wiley & Sons, Inc.; 2009.Google Scholar
- 10.World Health Organization. International histological classification of tumours. 2nd ed. Geneva: World Health Organization, 1969–1981; Berlin: Springer-Verlag, 1988–Present.Google Scholar
- 12.Paraskevopoulou MD, Georgakilas G, Kostoulas N, Vlachos IS, Vergoulis T, Reczko M, Filippidis C, Dalamagas T, Hatzigeorgiou AG. DIANA-microT web server v5.0: service integration into miRNA functional analysis workflows. Nucleic Acids Res. 2013 Jul;41(Web Server issue):W169-73. Available at http://diana.cslab.ece.ntua.gr/pathways/ 2013.
- 19.Haque I, Banerjee S, Mehta S, De A, Majumder M, Mayo MS, et al. Cysteine-rich 61-connective tissue growth factor-nephroblastoma-overexpressed 5 (CCN5)/Wnt-1-induced signaling protein-2 (WISP-2) regulates microRNA-10b via hypoxia-inducible factor-1α-TWIST signaling networks in human breast cancer cells. J Biol Chem. 2011;286(50):43475–85. doi: 10.1074/jbc.M111.284158.CrossRefPubMedPubMedCentralGoogle Scholar