Tumor Biology

, Volume 36, Issue 6, pp 4235–4241 | Cite as

Expression of Kindlin-1 in human hepatocellular carcinoma and its prognostic significance

  • Hua-Xing Ma
  • Qing-Hua Shu
  • Jing-Jing Pan
  • Dong Liu
  • Ge-Liang Xu
  • Jian-Sheng Li
  • Jin-Liang Ma
  • Wei-Dong Jia
  • Ji-Hai Yv
  • Yong-Sheng Ge
Research Article

Abstract

Kindlin-1 is a member of the Kindlin family of focal adhesion proteins and is implicated in cell adhesion, proliferation, polarity, and motility. Although expression of Kindlin-1 has recently been reported in a variety of human cancers, studies on its expression in human hepatocellular carcinoma (HCC) are currently lacking. This study aimed to determine the clinicopathological parameters and prognostic value of Kindlin-1 in HCC patients after surgical resection. The messenger RNA (mRNA) and protein levels of Kindlin-1 in 22 matched HCC specimens were assessed by quantitative real-time PCR (qRT-PCR) and Western blotting assays. The clinical and prognostic significance of Kindlin-1 in 68 cases of HCC was determined by immunohistochemistry. Kindlin-1 expression was higher in HCC tumor tissues relative to that in adjacent normal tissue at the both mRNA and protein levels (p < 0.05). Immunohistochemical results revealed that overexpression of Kindlin-1 was detected in 37 of 68 (54.4 %) tumor tissues and in seven of 68 (10.3 %) adjacent non-tumor tissues (p < 0.05). Positive Kindlin-1 expression was significantly correlated with tumor size, tumor capsula, status of metastasis, and tumor-node-metastasis (TNM) stage. Additionally, Kaplan–Meier survival analysis showed that positive Kindlin-1 expression was associated with unfavorable overall survival (OS) and disease-free survival (DFS). Multivariate analysis identified Kindlin-1 as an independent prognostic predictor for OS and DFS in HCC patients (p = 0.041 and 0.027, respectively). Taken together, our data suggest that Kindlin-1 could play an important role in HCC and might serve as a promising prognostic marker and potential target for HCC therapy.

Keywords

Kindlin-1 Hepatocellular carcinoma Prognostic marker Immunohistochemistry 

Notes

Acknowledgments

This research was supported by the Anhui Provincial Natural Science Foundation (No. 1308085MH147).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Hua-Xing Ma
    • 1
  • Qing-Hua Shu
    • 1
  • Jing-Jing Pan
    • 1
  • Dong Liu
    • 1
  • Ge-Liang Xu
    • 1
  • Jian-Sheng Li
    • 1
  • Jin-Liang Ma
    • 1
  • Wei-Dong Jia
    • 1
  • Ji-Hai Yv
    • 1
  • Yong-Sheng Ge
    • 1
  1. 1.Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery and Department of Hepatic Surgery, Anhui Provincial HospitalAnhui Medical UniversityHefeiChina

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