Abstract
MicroRNAs (miRNAs) are essential to the progression of osteosarcoma. Previous research using osteosarcoma samples confirmed that miR-377 expression is less than that observed in normal human osteoblast expression. These data suggest a role for miR-377 in osteosarcoma that warrants investigation. To address this concept, we measured miR-377 expression in two cell models, and we also observed that miR-377 was less expressed in osteosarcoma MG-63 cells compared to the hFOB1.19 human fetal osteoblastic cell line. Moreover, miR-377 overexpression reduced cell proliferation and suppressed invasion of MG-63 cells but had no effect on MG-63 cell apoptosis. Because cyclin-dependent kinase 6 (CDK6) may be a potential target of miR-377 in osteosarcoma cells, we overexpressed CDK6 and observed that overexpression attenuated tumor suppressive effects of miR-377 on cell proliferation. Our data suggest that miR-377 can suppress proliferation in MG-63 cells in part by targeting CDK6.
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Acknowledgments
This study was supported by grants from the Fundamental Research Funds for the Central Universities (No. 21614308) and the Medical Science Research Foundation of Guangdong Province (No. B2012195). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
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Liang Wang and Jianli Shao contributed equally to this paper.
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Wang, L., Shao, J., Zhang, X. et al. microRNA-377 suppresses the proliferation of human osteosarcoma MG-63 cells by targeting CDK6. Tumor Biol. 36, 3911–3917 (2015). https://doi.org/10.1007/s13277-014-3034-2
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DOI: https://doi.org/10.1007/s13277-014-3034-2