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Tumor Biology

, Volume 36, Issue 5, pp 3863–3870 | Cite as

Effect of HOTAIR rs920778 polymorphism on breast cancer susceptibility and clinicopathologic features in a Turkish population

  • Süleyman Bayram
  • Ahmet Taner Sümbül
  • Celal Yücel Batmacı
  • Ahmet Genç
Research Article

Abstract

Overexpression of Hox transcript antisense intergenic RNA (HOTAIR), a long non-coding RNA (lncRNA), is associated with cancer cell proliferation, invasion, progression, and metastasis as well as poor survival in a variety of human cancers including breast cancer (BC). A common functional single nucleotide polymorphism (SNP) rs920778 (T → C) in the intronic enhancer of the HOTAIR has been reported to influence HOTAIR expression and cancer predisposition, but the association of HOTAIR rs920778 polymorphism with BC susceptibility and clinicopathological features has yet to be investigated. We genotyped HOTAIR rs920778 polymorphism in 245 Turkish women including 123 BC patients and 122 age-matched healthy controls by a real-time polymerase chain reaction (PCR) with the TaqMan assay. We found that the CC genotype of HOTAIR rs920778 polymorphism significantly increased the risk of BC in both codominant (odds ratio (OR) = 2.12, 95 % confidence interval (CI) 1.00–4.51, P = 0.05) and recessive (OR = 2.40, 95 % CI 1.22–4.73, P = 0.01) inheritance genetic models. Our research also indicated an association between the CC genotype of HOTAIR rs920778 polymorphism and clinicopathologic features of tumor, including advanced tumor–node–metastasis (TNM) stage, larger tumor size, distant metastasis, and poor histological grade (P < 0.05). Because our findings suggest for the first time that the CC genotype of HOTAIR rs920778 polymorphism might play important roles in genetic susceptibility to BC development and aggressiveness in a Turkish population, further independent studies are required to validate our findings in a larger series, as well as in patients of different populations.

Keywords

Breast cancer HOTAIR lncRNA HOTAIR rs920778 polymorphism Genetic susceptibility Clinicopathologic features 

Notes

Acknowledgments

The authors thank all the subjects who participated in this study.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Süleyman Bayram
    • 1
  • Ahmet Taner Sümbül
    • 2
  • Celal Yücel Batmacı
    • 3
  • Ahmet Genç
    • 4
  1. 1.Department of Nursing, Adıyaman School of HealthAdıyaman UniversityAdıyamanTurkey
  2. 2.Department of Medical Oncology, Medical FacultyMustafa Kemal UniversityHatayTurkey
  3. 3.Department of Internal Medicine, Medical FacultyMustafa Kemal UniversityHatayTurkey
  4. 4.Vocational School of Health ServicesAdıyaman UniversityAdıyamanTurkey

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