Tumor Biology

, Volume 36, Issue 5, pp 3611–3619 | Cite as

HOTAIR enhanced aggressive biological behaviors and induced radio-resistance via inhibiting p21 in cervical cancer

  • Li Jing
  • Wang Yuan
  • Dong Ruofan
  • Yu Jinjin
  • Qiu Haifeng
Research Article

Abstract

We previously reported the frequent overexpression of HOX Antisense Intergenic RNA (HOTAIR) in human cervical cancer, which was significantly correlated with tumor progression and poor prognosis. In the present study, we investigated the detailed biological functions of HOTAIR in cervical cancer. In vitro, upregulation of HOTAIR inhibited apoptosis and promoted cellular proliferation, cell cycle progression, migration, and invasion; on the contrast, downregulation of HOTAIR induced more apoptosis, suppressed cellular proliferation, cell cycle, migration, and invasion. Moreover, a high level of HOTAIR was notably associated with radio-resistance and downregulation of p21 in the primary cultured cervical cancer cells. Further, we demonstrated that elevated HOTAIR could induce radio-resistance via inhibiting p21 in HeLa cells, while knockdown of HOTAIR upregulated p21 and consequentially increased the radio-sensitivity of C33A cells. Consistently, stable knockdown of HOTAIR significantly suppressed tumor growth and sensitized cervical cancer to radiotherapy in vivo. In conclusion, HOTAIR served as an onco-lncRNA in cervical cancer which could enhance various aggressive biological behaviors. Moreover, we proved that HOTAIR execute its functions mainly through inhibiting the p21 expression. These results proposed that targeting HOTAIR might be a potent therapeutic strategy in cervical cancer, especially for those patients who accepted radiotherapy.

Keywords

Cervical cancer HOTAIR p21 Cell cycle Radio-resistance 

Notes

Acknowledgments

Our study was supported by grants from the Scientific Bureau of Wuxi (CSE31N1418) and the Health Bureau of Wuxi (MS201417, FYKY201401) to Yuan Wang.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Li Jing
    • 1
    • 2
  • Wang Yuan
    • 3
  • Dong Ruofan
    • 3
  • Yu Jinjin
    • 3
  • Qiu Haifeng
    • 2
    • 4
  1. 1.Department of OncologyThe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Institute of Clinical MedicineThe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  3. 3.Department of Obstetrics and GynecologyThe Affiliated Hospital of Jiangnan University and The 4th People’s Hospital of WuxiWuxiChina
  4. 4.Department of Obstetrics and GynecologyThe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

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