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Tumor Biology

, Volume 36, Issue 5, pp 3521–3531 | Cite as

HOXA5 indicates poor prognosis and suppresses cell proliferation by regulating p21 expression in non small cell lung cancer

  • Mei-ling Zhang
  • Feng-qi Nie
  • Ming Sun
  • Rui Xia
  • Min Xie
  • Kai-hua Lu
  • Wei Li
Research Article

Abstract

Homeobox genes, a superfamily of evolutionarily conserved developmental genes, function as critical master regulatory factors in controlling body plan specification and cell fate determination. Recently, a substantial body of evidence indicates that the aberrant Homeobox (HOX) genes also play key roles in the development of cancers. Many reports have shown not only that HOX gene expression is upregulated or downregulated in many cancers but also that the expression of specific HOX genes tends to differ based on tissue type. Homeobox A5 (HOXA5) is a master regulator of the morphogenesis and cell differentiation, and its expression is also downregulated in many cancers mediated by DNA methylation. However, its biological role and clinical significance in nonsmall cell lung cancer (NSCLC) development and progression are not well documented. In this study, we found that expression levels of HOXA5 were significantly decreased in NSCLC tissues compared with adjacent normal tissues. Its expression level was significantly correlated with tumor–node–metastasis (TNM) stages, tumor size, and lymph node metastasis. Moreover, patients with lower levels of HOXA5 expression had a relatively poor prognosis. Furthermore, ectopic overexpression of HOXA5 could inhibit cell proliferation and invasion, while knockdown HOXA5 by siRNA promoted cell proliferation in NSCLC cells partly via regulating p21 expression. Our findings present that decreased HOXA5 could be identified as a poor prognostic biomarker in NSCLC and regulate cell proliferation and invasion.

Keywords

NSCLC Homeobox genes HOXA5 Proliferation p21 

Notes

Acknowledgments

This work was supported by the National Natural Scientific Foundation of China (No. 81372397 to Kai-hua Lu, No. 81302012 to Wei Li) and the Priority Academic Program Development of Jiangsu Higher Education Institutions (JX10231801).

Conflicts of interest

None

Supplementary material

13277_2014_2988_MOESM1_ESM.xls (10 kb)
Supplementary Table 1 (XLS 10 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  1. 1.Department of OncologyFirst Affiliated Hospital, Nanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.Department of Biochemistry and Molecular BiologyNanjing Medical UniversityNanjingPeople’s Republic of China

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