Tumor Biology

, Volume 36, Issue 5, pp 3237–3243 | Cite as

Inhibitory effect of ibuprofen on tumor survival and angiogenesis in gastric cancer cell

Research Article

Abstract

Numerous epidemiological studies have suggested effectiveness of long-term and regular use of non-steroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen and aspirin, in preventing and treatment of certain cancers including prostate, colon, breast, lung, and gastric cancers. We have studied the potential anti-turmeric effect of ibuprofen in adenocarcinoma gastric cell line (AGS). The effects of ibuprofen were investigated on cell proliferation, apoptosis, angiogenesis, and expression of stemness marker genes using real-time RT-PCR, DNA laddering, and tube formation assays via ECM gel and human umbilical vein endothelial cells (HUVECs). Annexin-V-FLUOS and propidium iodide (PI) were used to stain the apoptotic cells. Our findings indicate that ibuprofen at the concentrations of 100, 200, 300, 400, and 500 μM is able to reduce the cancerous characteristics of the AGS cells by inducing apoptosis, inhibition of cell proliferation, and angiogenesis. Real-time RT-PCR showed that ibuprofen altered the expression of several genes including Akt, P53, PCNA, Bax, and Bcl2 in the AGS cells. In addition, reduction in CD44 and OCT3/4 transcript levels revealed that ibuprofen reduces the stemness of the AGS cells and therefore it could be used as a potential anti-tumor drug.

Keywords

Angiogenesis Apoptosis Adenocarcinoma gastric cell line (AGS) Gastric cancer Ibuprofen 

Notes

Acknowledgments

The authors are grateful to Fatemeh Mahmoodi and Kamran Mansouri for their contribution in completing this work. We would like to thank Dr. Sajjad Sisakhtnezhad for reviewing the manuscript.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Hassan Akrami
    • 1
  • Saman Aminzadeh
    • 1
  • Hossein Fallahi
    • 1
  1. 1.Department of Biology, Faculty of ScienceRazi UniversityKermanshahIran

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