Tumor Biology

, Volume 36, Issue 4, pp 2913–2919 | Cite as

Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway

  • Fubin Zhang
  • Xiaowei Song
  • Li Li
  • Jingfang Wang
  • Leyuan Lin
  • Cong Li
  • Hongtao Li
  • Yanju Lv
  • Yinghua Jin
  • Ying Liu
  • Yu Hu
  • Tao Xin
Research Article

Abstract

One purified polysaccharide protein tyrosine phosphatase (PTP) was isolated from the roots of Polygala tenuifolia. The aim of the present study is to investigate the antitumor effect of PTP on human ovarian cancer OVCAR-3 cells and explore the molecular mechanism of the action involved. The results of MTT assay and apoptosis detection assay showed that PTP inhibited cellular proliferation of OVCAR-3 cells and induced apoptotic cellular death via arresting cell circle at the G0/G1 phase. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis identified that bcl-2 gradually decreased at both transcription and protein levels after PTP treatment for 48 h in OVCAR-3 cells, while those of bax, cytochrome c, caspase-3, and caspase-9 increased. In addition, the low expression of NF-κB in PTP-treated OVCAR-3 cells would trigger the extrinsic pathway of programmed cell death signaling in tumor cells. These results together suggest that PTP may induce apoptosis of OVCAR-3 cells through a mitochondrial pathway.

Keywords

Polygala tenuifolia Polysaccharide Apoptosis Mitochondrial Caspase Ovarian cancer 

Notes

Acknowledgments

This research is funded by the National Natural Science Foundation of China (no. 81373904) and the Doctoral Research Fund of the Second Affiliated Hospital of Harbin Medical University (Harbin, China).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Fubin Zhang
    • 1
  • Xiaowei Song
    • 2
  • Li Li
    • 2
  • Jingfang Wang
    • 2
  • Leyuan Lin
    • 3
  • Cong Li
    • 4
  • Hongtao Li
    • 5
  • Yanju Lv
    • 2
  • Yinghua Jin
    • 2
  • Ying Liu
    • 2
  • Yu Hu
    • 2
  • Tao Xin
    • 2
  1. 1.Department of Gynecology, The Third Affiliated (Tumor) HospitalHarbin Medical UniversityHarbinChina
  2. 2.Department of Medical Oncology, The Second Affiliated HospitalHarbin Medical UniversityHarbinChina
  3. 3.The 209 Hospital of People’s Liberation Army (PLA)MudanjiangChina
  4. 4.Department of Pathology, The Third Affiliated (Tumour) HospitalHarbin Medical UniversityHarbinChina
  5. 5.Department of Molecular MedicineMayo ClinicRochesterUSA

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