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Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway

  • Research Article
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Tumor Biology

Abstract

One purified polysaccharide protein tyrosine phosphatase (PTP) was isolated from the roots of Polygala tenuifolia. The aim of the present study is to investigate the antitumor effect of PTP on human ovarian cancer OVCAR-3 cells and explore the molecular mechanism of the action involved. The results of MTT assay and apoptosis detection assay showed that PTP inhibited cellular proliferation of OVCAR-3 cells and induced apoptotic cellular death via arresting cell circle at the G0/G1 phase. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis identified that bcl-2 gradually decreased at both transcription and protein levels after PTP treatment for 48 h in OVCAR-3 cells, while those of bax, cytochrome c, caspase-3, and caspase-9 increased. In addition, the low expression of NF-κB in PTP-treated OVCAR-3 cells would trigger the extrinsic pathway of programmed cell death signaling in tumor cells. These results together suggest that PTP may induce apoptosis of OVCAR-3 cells through a mitochondrial pathway.

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Acknowledgments

This research is funded by the National Natural Science Foundation of China (no. 81373904) and the Doctoral Research Fund of the Second Affiliated Hospital of Harbin Medical University (Harbin, China).

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Authors and Affiliations

  1. Department of Gynecology, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin, 150040, China

    Fubin Zhang

  2. Department of Medical Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China

    Xiaowei Song, Li Li, Jingfang Wang, Yanju Lv, Yinghua Jin, Ying Liu, Yu Hu & Tao Xin

  3. The 209 Hospital of People’s Liberation Army (PLA), Mudanjiang, 157000, China

    Leyuan Lin

  4. Department of Pathology, The Third Affiliated (Tumour) Hospital, Harbin Medical University, Harbin, 150040, China

    Cong Li

  5. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, 55905, USA

    Hongtao Li

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  1. Fubin Zhang
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  2. Xiaowei Song
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  3. Li Li
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  4. Jingfang Wang
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  5. Leyuan Lin
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  9. Yinghua Jin
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  11. Yu Hu
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  12. Tao Xin
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Correspondence to Tao Xin.

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Zhang, F., Song, X., Li, L. et al. Polygala tenuifolia polysaccharide PTP induced apoptosis in ovarian cancer cells via a mitochondrial pathway. Tumor Biol. 36, 2913–2919 (2015). https://doi.org/10.1007/s13277-014-2921-x

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  • DOI: https://doi.org/10.1007/s13277-014-2921-x

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