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Different frequencies and effects of ABCB1 T3435C polymorphism on clinical and laboratory features of B cell chronic lymphocytic leukemia in Kurdish patients

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Tumor Biology

Abstract

Finding the effects of gene polymorphism on cancer pathogenesis is very desirable. The ATP-binding cassette is involved in drug metabolism, and the polymorphism of this gene may be an important risk factor in B cell chronic lymphocytic leukemia (B-CLL) or progression and/or response to chemotherapy agents. For the first time, the present study was aimed to evaluate the probable effects of ABCB1 T3435C polymorphism on clinical and laboratory features of Kurdish patients with B-CLL. This descriptive analytical case-control study was performed on 50 B-CLL patients and 100 healthy subjects. Serum levels of beta-2-microglobulin (B2M) and lactate dehydrogenase (LDH) and blood WBC, RBC, Plt and ESR were measured. The T3435C polymorphism of the ABCB1 gene was determined by PCR-RFLP. Concentration of serum and blood markers was significantly higher in the malignant group than in the benign subjects. The CC genotype had the highest frequency (66 %) in the patient groups. There are no significant differences between the genotypes and type of treatment. Our results demonstrate the high frequency of C allele of ABCB1 T3435C in B-CLL patients with Kurdish ethnicity. We also show that this polymorphism has a significant risk factor in B-CLL. However, the effect of this polymorphism on clinical and laboratory characteristics of B-CLL patients was not significant.

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Acknowledgments

The authors wish to thank all patients and health staff who participated in this study. This work has no financially support.

Conflicts of interest

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Authors and Affiliations

  1. Department of Biochemistry, College of Science, Kurdistan Science and Research Branch, Islamic Azad University, Sanandaj, Iran

    Farzad Maroofi

  2. Department of Biology, College of Science, Islamic Azad University, Sanandaj, Iran

    Sabrieh Amini

  3. Department of Epidemiology and Biostatistics, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Daem Roshani

  4. Department of Internal Medicine, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Bayazid Ghaderi

  5. Cellular and Molecular Research Center, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

    Mohammad Abdi

  6. Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Room No. 384, Pasdaran Boulevard, Sanandaj, Iran

    Mohammad Abdi

  7. Division of Hematology-Oncology, Tohid Hospital, Geryashan Boulevard, Sanandaj, Iran

    Bayazid Ghaderi

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  1. Farzad Maroofi
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  2. Sabrieh Amini
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Correspondence to Bayazid Ghaderi or Mohammad Abdi.

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Maroofi, F., Amini, S., Roshani, D. et al. Different frequencies and effects of ABCB1 T3435C polymorphism on clinical and laboratory features of B cell chronic lymphocytic leukemia in Kurdish patients. Tumor Biol. 36, 2863–2868 (2015). https://doi.org/10.1007/s13277-014-2914-9

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  • DOI: https://doi.org/10.1007/s13277-014-2914-9

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