Tumor Biology

, Volume 36, Issue 4, pp 2831–2844 | Cite as

LASS2/TMSG1 inhibits growth and invasion of breast cancer cell in vitro through regulation of vacuolar ATPase activity

  • Fang Mei
  • Jiangfeng You
  • Beiying Liu
  • Mengxue Zhang
  • Jiangying Liu
  • Bo Zhang
  • Fei Pei
Research Article


Homo sapiens longevity assurance homologue 2 of yeast LAG1 (LASS2)/tumor metastasis suppressor gene 1 (TMSG1) was a novel tumor metastasis-related gene identified using messenger RNA differential display from non-metastatic human prostate cancer cell variants. The mechanism of LASS2/TMSG1 inhibiting tumor invasion metastasis in breast cancer cells had not been well investigated. In the present study, a full length of 1.2 kb LASS2/TMSG1 complementary DNA (cDNA) coding for a protein of 380 amino acids was cloned. PcDNA3 eukaryotic expression plasmids of LASS2/TMSG1 were constructed and transfected into human breast cancer cell line MCF-7 by lipofectin transfection method. And, the biological effects were observed comparing with control groups. As the result, LASS2/TMSG1 inhibited cell growth in vitro by increasing apoptosis and changing cell cycle distribution. Furthermore, the vacuolar ATPase (V-ATPase) activity and extracellular hydrogen ion concentration were significantly decreased and the activity of secreted matrix metalloproteinase-2 (MMP-2) was downregulated in MCF-7 cells overexpressing LASS2/TMSG1 compared with the controls. Therefore, LASS2/TMSG1 may inhibit growth and invasion of breast cancer cell in vitro through decreasing V-ATPase activity and extracellular hydrogen ion concentration and inactivating secreted MMP-2. The findings provided the evidence that the LASS2/TMSG1 gene had tumor growth and invasion suppressor function in human breast cancer cell and may provide a promising target for cancer metastasis diagnosis and therapy.


LASS2/TMSG1 Breast cancer Vacuolar ATPase MMP-2 



The article was supported by the National Sciences Foundation of China (30971142). Thanks to Beiying Liu for data analysis and figure modification.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2014

Authors and Affiliations

  • Fang Mei
    • 1
  • Jiangfeng You
    • 1
  • Beiying Liu
    • 2
  • Mengxue Zhang
    • 1
  • Jiangying Liu
    • 1
  • Bo Zhang
    • 1
  • Fei Pei
    • 1
  1. 1.Department of Pathology, School of Basic Medical SciencesPeking University Health Science CenterBeijingChina
  2. 2.School of Mechanical EngineeringUniversity of Science & Technology BeijingBeijingChina

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